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Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K4) antibody (ab71998)
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Western blot - Histone H3 (tri methyl K4) antibody (ab71998)
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ChIP - Histone H3 (tri methyl K4) antibody (ab71998)
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Product Name
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Histone H3 (tri methyl K4) antibody
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See all Histone H3 (methylated) antibodies (36)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (tri methyl K4)
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Immunogen
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A synthetic peptide containing RT[Me3-K]QT in which Me3-K corresponds to trimethyl lysine 4 of human histone H3.
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Reacts with
(species key)
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Hu, Ms, Rat, At, Chk, Cow, Dm, Xl
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Specificity
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ab71998 is specific for Histone H3 tri-methylated at K4. It does not cross react to nonmethylated H3 or mono-, di-methylated Histone H3.
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Tested applications
(see key)
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ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
ICC/IF: 1/100.
IP: Use at a concentration of 2-5 µg/ml.
WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
This antibody is not ChIP grade. Results shown in the image indicate a slight enrichment at heterchromatic loci. This suggests the antibody may exhibit some cross-reactivity with histone modifications localized at these regions. We would welcome feedback about this antibodies performance in ChIP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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3T3 Nuclear Extract and 3T3 cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> H3 >> Methylated
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Relevance
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Histones are basic nuclear proteins that, together with DNA, make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer, which is composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin structure is further compacted through the interaction of a linker histone H1.
Methylation of specific residues in the histone tails is a central modification for regulating epigenetic transitions in chromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, whilst arginine residues can be mono- or di-methylated.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2 containing antibody stabilizer
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Purification notes
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ab71998 is purified by Site-modification specific Affinity Chromatography.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (tri methyl K4) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (tri methyl K4) antibody images, references, products related to ab71998 and other tools.
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Histone H3 (tri methyl K4) antibody images:
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 Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K4) antibody (ab71998)
ab71998 at 1/100 dilution staining Histone H3 in 3T3 cells by Immunocytochemistry/ Immunofluorescence. A FITC labeled goat anti Rb IgG was used for visualization under fluorescent microscope.
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Western blot - Histone H3 (tri methyl K4) antibody (ab71998)
All lanes : Histone H3 (tri methyl K4) antibody (ab71998) at 1/1000 dilution
Lane 1 : 3T3 nuclear extract immunoprecipitated
using 4 µg ab71998
Lane 2 : 3T3 nuclear extract immunoprecipitated
using control Rabbit IgG
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa (possible IgG),50 kDa (possible IgG).
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ChIP - Histone H3 (tri methyl K4) antibody (ab71998)
Chromatin was prepared from Jurkat cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab71998 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 (tri methyl K4) antibody - more information
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Histone H3 (tri methyl K4) antibody - other tools:
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