Products:Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
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ab46666 |
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ab4627 has been referenced in 7 publications.
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Lanes 1 & 3 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/500 dilution
Lanes 2 & 4 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/1000 dilution
Lane 1 : Calf thymus histone prep.
Lane 2 : Calf thymus histone prep.
Lane 3 : Calf thymus histone prep. with
Lane 4 : Calf thymus histone prep. with
Lysates/proteins at 1 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size : 12 kDa
Observed band size : 11 kDa (why is the actual band size different from the predicted?)
Predicted Molecular weight11KDa
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4627 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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