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RabMAbs (1808-1)

Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)

Abreviews (2)Q&A (4)Specific References (7)

Overview

  • Product nameAnti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP GradeSee all Histone H4 (acetylated) primary antibodies ...
  • Description
    Rabbit monoclonal [EP1000Y] to Histone H4 (acetylated) (acetyl K5) - ChIP Grade
  • Tested applicationsChIP, WB, IHC-P, ICC/IF, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rice
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human Histone H4.

  • Positive controlHeLa cells or human brain glioma.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab51997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ChIP ChIP: Use at an assay dependent dilution. See reference.
WB WB: 1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P IHC-P: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/250 - 1/500.
IP IP: 1/50.
  • Application notesIs unsuitable for or Flow Cyt.

    • Target

    • Alternative names
        H4 antibodyH4_HUMAN antibodyH4F2 antibody
        HIST4H4 antibodyHistone H4 antibody
      see all

    Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade images

    • Western blot - Histone H4 (acetyl K5) antibody [EP1000Y] (ab51997)
      Western blot - Histone H4 (acetyl K5) antibody [EP1000Y] (ab51997)
      All lanes : Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997) at 1/1000000 dilution

      Lane 1 : Untreated HeLa cells
      Lane 2 : TSA treated HeLa calls

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labelled (1:2000)

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunohistochemistry (Paraffin-embedded sections) - Histone H4 (acetyl K5) antibody [EP1000Y] (ab51997)
      Immunohistochemistry (Paraffin-embedded sections) - Histone H4 (acetyl K5) antibody [EP1000Y] (ab51997)
      Ab51997 (1:500) staining human histone H4 on human brain glioma by immunohistochemistry using paraffin embedded tissue.
    • Immunocytochemistry/ Immunofluorescence - Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)
      Immunocytochemistry/ Immunofluorescence - Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)
      ICC/IF image of ab51997 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51997, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)

    This product has been referenced in:
    • Zhang X  et al. Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor a target gene activation. Proc Natl Acad Sci U S A 109:13331-6 (2012). ICC/IF ; Mouse . Read more (PubMed: 22853951) »
    • Sirbu BM  et al. Analysis of protein dynamics at active, stalled, and collapsed replication forks. Genes Dev 25:1320-7 (2011). Read more (PubMed: 21685366) »

    See all 7 Publications for this product

    Publishing research using ab51997 ? Please let us know so that we can cite the reference in this datasheet

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade

     Excellent
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Xenopus laevis Cell (Fibroblast)
    Specification Fibroblast
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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    Verified customer

    Submitted Apr 05 2013


    Thanks for your help with that order. I have another question for an additional project we are initiating requiring lots of different antibodies. This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified...

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    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    We ordered ab87277 Anti-RPA32/RPA2 (phospho S4 + S8) antibody. We are still deciding if we want to evaluate ab88675. When we complete our test we will submit the results to you. Thank you

    Thank you for your reply. Please keep me updated on what you decided about ab88675. I have created a discount code for you to test ab87277 in Western blot, please see below for details and let me know if you have any questions! DISCOUNT CODE: *** Expirati...

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    Ab105362 and ab11169 gave very weak/no signal in Western blot with 3T3 lysate. Would like information about the testing discount program.

    Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending free of charge vials of ab51997 and ab7311 on the order *** which should have arrived on November 23.    Since ab87277 and ab88675 ha...

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    Ab105362 and ab11169 gave very weak/no signal in Western blot with 3T3 lysate. Would like information about the testing discount program.

    Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending free of charge vials of ab51997 and ab7311 on the order *** which should arrive tomorrow. Since ab87277 and ab88675 have not been tes...

    Read More

    Western blot abreview for Anti-Histone H4 (acetylated) (acetyl K5) antibody [EP1000Y] - ChIP Grade

     Below Average
    Abreviews
    Application Western blot
    Sample Schizosaccharomyces pombe Purified protein (Purified modified histone peptides)
    Loading amount 0.1 µg
    Specification Purified modified histone peptides
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Verified customer

    Submitted Feb 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"