You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-Histone H4 antibodySee all Histone H4 primary antibodies ...
  • Description
    Rabbit polyclonal to Histone H4 - ChIP Grade
  • Tested applicationsIP, ChIP, IHC-P, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis, Fruit fly (Drosophila melanogaster)
    Predicted to work with: Chicken, Cow, Pig, Caenorhabditis elegans
  • Immunogen

    Synthetic peptide derived from within residues 50 to the C-terminus of Human Histone H4.

    Read Abcam's proprietary immunogen policy

    (Peptides available as ab13843 and ab13843.)

  • Positive controlThis antibody gave a positive control in the following lysates: Histone prep HeLa Histone This antibody gave a positive control in the following mouse lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell MEF1 (Mouse embryonic fibroblast cell line) Whole Cell This antibody gave a positive control in the following rat lysate: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab10158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent concentration.
ChIP ChIP: Use 2 µg for 25 µg of chromatin.
IHC-P IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF ICC/IF: 1/200.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).Can be blocked with Histone H4 peptide (ab13843).

Target

  • Alternative names
      dJ160A22.1 antibodydJ160A22.2 antibodydJ221C16.1 antibody
      dJ221C16.9 antibodyFO108 antibodyH4 antibodyH4.k antibodyH4/a antibodyH4/b antibodyH4/c antibodyH4/d antibodyH4/e antibodyH4/g antibodyH4/h antibodyH4/I antibodyH4/j antibodyH4/k antibodyH4/m antibodyH4/n antibodyH4/p antibodyH4_HUMAN antibodyH4F2 antibodyH4F2iii antibodyH4F2iv antibodyH4FA antibodyH4FB antibodyH4FC antibodyH4FD antibodyH4FE antibodyH4FG antibodyH4FH antibodyH4FI antibodyH4FJ antibodyH4FK antibodyH4FM antibodyH4FN antibodyH4M antibodyHIST1H4A antibodyHIST1H4B antibodyHIST1H4C antibodyHIST1H4D antibodyHIST1H4E antibodyHIST1H4F antibodyHIST1H4H antibodyHIST1H4I antibodyHIST1H4J antibodyHIST1H4K antibodyHIST1H4L antibodyHIST2H4 antibodyHIST2H4A antibodyHIST4H4 antibodyHistone 1 H4a antibodyHistone 1 H4b antibodyHistone 1 H4c antibodyHistone 1 H4d antibodyHistone 1 H4e antibodyHistone 1 H4f antibodyHistone 1 H4h antibodyHistone 1 H4i antibodyHistone 1 H4j antibodyHistone 1 H4k antibodyHistone 1 H4l antibodyHistone 2 H4a antibodyhistone 4 H4 antibodyHistone H4 antibodyMGC24116 antibody
    see all

Anti-Histone H4 antibody images

  • Western blot - Histone H4 antibody (ab10158)
    Western blot - Histone H4 antibody (ab10158)
    All lanes : Anti-Histone H4 antibody (ab10158) at 1 µg/ml

    Lane 1 : Histone prep
    Lane 2 : HeLa histone lysate
    Lane 3 : Histone prep with Histone H4 peptide (ab13843) at 1 µg/ml
    Lane 4 : HeLa Histone lysate with Histone H4 peptide (ab13843) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • Western blot - Histone H4 antibody (ab10158)
    Western blot - Histone H4 antibody (ab10158)
    All lanes : Anti-Histone H4 antibody (ab10158) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • ChIP - Anti-Histone H4 antibody (ab10158)
    ChIP - Anti-Histone H4 antibody (ab10158)
    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab10158 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
  • Immunoprecipitation - Anti-Histone H4 antibody (ab10158)
    Immunoprecipitation - Anti-Histone H4 antibody (ab10158)
    Histone H4 was immunoprecipitated using 0.5mg NIH3T3 whole cell lysate, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10158.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 14kDa: Histone H4; non specific - 52 and 85kDa: We are unsure as to the identity of this extra band.
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody (ab10158)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody (ab10158)This image is courtesy of an anonymous Abreview
    ab10158 staining Histone H4 in Mouse thymocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/150 in PBS) for 2.5 hours at 25°C. An Oregon Green®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody (ab10158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody (ab10158)
    IHC image of Histone H4 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10158, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Histone H4 antibody (ab10158)

This product has been referenced in:
  • Franklin S  et al. Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth. Mol Cell Proteomics 11:M111.014258 (2012). WB . Read more (PubMed: 22270000) »
  • Bowman A  et al. The histone chaperones nap1 and vps75 bind histones h3 and h4 in a tetrameric conformation. Mol Cell 41:398-408 (2011). Read more (PubMed: 21329878) »

See all 22 Publications for this product

Publishing research using ab10158 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab10158 Submit a Question for ab10158

Displaying 1 - 10 of 50 results for Abreviews and Q&A

10
Votes: Highest First

Western blot

 Good
Abreviews
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Erythrocytes)
Loading amount 50000 cells
Specification Erythrocytes
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
abreview image
Username

Verified customer

Submitted Apr 05 2013

Ich möchte humanes Histone 4 (C-terminus) in einem bead-based sandwich immunoassay testen.

Können Sie mir alternative Histone H4 (C-terminus) Antikörper empfehlen, welche in so einem Setting als Paar funktionieren wuerden?



Leider haben wir kein Antikörperpaar, welches für Ihre Zwecke geeignet ist, da sich entweder die Immunogensequenzen zu sehr überschneiden, oder aber die Applikation nicht funktioniert.

Sie können sich aber noch an di...

Read More

Is acetylated histone H4 recognized by this antibody? I used 10158 antibody to detect histone H4 level in Arabidopsis. H4 can be recognized in wild type, but not in my mutant. On the other hand, Acetylated H4 can be recognized by specific AcH4 antibody in...

Read More

The antibody ab10158 should be capable of detecting acetylated H4. It is raised against an amino acid sequence that has one acetylation site, K91. The antibody is a polyclonal antibody, though, so we expect it binds to multiple epitopes, even if k91 is ac...

Read More

I have been using tubulin ab 15246 at 1:200 dilution for Drosophila tissues and it works well for western blots using Supersignal west pico.



Would you be interested in submitting one of our online Abreviews? This will allow other researchers to see your protocol, and any data that you'd like to include, which I agree will be useful for others.

Hello,

I am still waiting for a response so that I may purchase this antibody.

The email below was initially sent on August 23. I'm sorry if it did not reach you on the first try. Please contact me if this is not the information you were expecting or if you have any further questions.

======


I am very pleas...

Read More

Ich habe einen Mix aus humaner und nicht-eukaryotischer DNA nach Sonication oder Electrolyse.

Wäre es möglich den Großteil der humanen DNA mittels eines anti-Histon Ab (z.B.: anti-H4 C-terminaler Ab) loszuwerden?

Eini...

Read More

Vielen Dank für Ihre Anfrage.

Da nicht-eukaryotische DNA (aus Prokaryoten in diesem Fall?) keine Histone enthalten sollten, denke ich dass Sie hier eine elegante Lösung gefunden haben. Ich würde Antikörper empfehlen, die entweder für die IP get...

Read More

Western blot

 Good
Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Non-reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Read More
Username

Verified customer

Submitted Aug 03 2012

Thank you very much for the generous offer. I gladly accept and will happily let you know the outcome. What I hope to do is test my distant eukaryote with a positive control species (like yeast) Could you perhaps also tell me (or offer a discount or a sam...

Read More

I am very pleased to hear you would like to accept our offer and test ab10158 in WB with a distant eukaryote. This code will give you: 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB with distant e...

Read More

Dear Abcam, I'm looking for antibodies to conserved regions of Histone H4. I am working on a eukaryote that is rather distant from animals, yeasts, and plants. So I thought that perhaps the quickest way to get confirmation that this unusual H4 would be to...

Read More

Thank you very much for your interest in ab10158.

To our knowledge, ab10158 has not been tested in a distant eukaryote. Therefore, I can offer a discount off a future purchase if you buy ab10158 now, test it indistant eukaryote and submit feedbac...

Read More

check sequence homology for ab1791 and ab10158 with wasp (nasonia vitripennis)
send discount codes

Thank you for your phone call.

I have checked the sequence homology for the wasp sequence with the human sequence for both Histone H3 and H4 and it is 96% and 99%, respectively.

DISCOUNT CODE: xxx for ab1791
DISCOUNT CODE: xxx for...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"