Overview

  • Product name
  • Description
    Rabbit polyclonal to Histone H4
  • Tested applications
    Suitable for: IP, ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 10-59. Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805 ) around the acetylation site of lysine 12 (L-G-KA-G-G).
    Database link: P62805

  • Positive control
    • Human breast carcinoma tissue and Jurkat cell extracts treated with TSA (400nM, 24hours).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • Histone H4 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab61255.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 13kDa; Histone H4

  • ab61255 at 1/50 - 1/100 dilution staining Histone H4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Histone H4 antibody (ab61255) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with TSA (400nM, 24hours)
    Lane 2 : Jurkat cell extracts treated with TSA (400nM, 24hours) with the immunising peptide


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ICC/IF image of ab61255 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61255, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Sadeghi L  et al. The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast. EMBO Rep 16:1673-87 (2015). WB ; Schizosaccharomyces pombe . Read more (PubMed: 26518661) »
  • Attia AS  et al. Analysis of the Staphylococcus aureus abscess proteome identifies antimicrobial host proteins and bacterial stress responses at the host-pathogen interface. Pathog Dis 69:36-48 (2013). Read more (PubMed: 23847107) »

See all 3 Publications for this product

Customer reviews and Q&As

Yes, the antibody can also recongize the acetylated histone H4. This antibody has been tested this antibody by ELISA and can react with Acetyl peptide and Non-acetyl peptide. The results are here below: Dilution 1:1000 - 1:4...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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