Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
- DNA RNA and cDNA
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade
See all Histone H4 products (10) ...
Description
Rabbit polyclonal to Histone H4 (mono methyl K20) - ChIP Grade
Specificity
This antibody is specific for the mono-methyl form of Histone H4.
Tested applications
IHC-Fr, WB, ICC/IF, Flow Cyt, CHIPseq, ChIP, IFmore details
Cross reactivity
Reacts with
Mouse, Cow, Human, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe
Predicted to work with
all Mammals
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H4, mono methylated at K20.
(Peptide available as ab170 43.)
Positive control
Calf Thymus Histone Preparation; Hela whole cell extract
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Interphase: The cells within the culture show a considerable variability in the intensity of staining with the antibody. The relationship between trimethylation levels and cell cycle have not yet been determined but may be a contributor to the amount of methylation detected in each cell. Heterochromatic regions of the interphase nucleus are the primary sites of trimethylation observed by indirect immunofluroescence. Mitosis: Discrete chromosomal regions are labelled intensely, with lower level fluorescence throughout the remainder of the chromosome arms Data from IF performed by Kirk McManus in the lab of Michael Hendzel as part of the antibody characterisation at cellnucleus.com
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H4 >> Methylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000.
ICC/IF: Use at an assay dependent dilution. Cautionary note: At low dilutions, the antibody may cross-react with microtubules. This can be minimized by increasing the dilution of the antibody when used in immunofluroescence.
Flow Cyt
Flow Cyt: 1/200.Flow Cyt: 1/200.
CHIPseq: Use at an assay dependent concentration. PubMed: 22196736
ChIP: Use 2-25 µg for µg of chromatin.
IF
IF: 1/250.IF: 1/250.
Target
Relevance
Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.
Cellular localization
Nuclear
Alternative names
- H4F2 antibody
- H4FN antibody
- HIST1H4 antibody
see all
Database links
- Entrez Gene: 45315 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 8370 Human
- Entrez Gene: 121504 Human
- Entrez Gene: 320332 Mouse
- GenBank: NM_003548 Human
- Omim: 142750 Human
- SwissProt: P02304 Human
- SwissProt: P62805 Human
see all
Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade images:
- Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
Rabbit polyclonal to Histone H4 mono methyl K20 at 1/1000 on histone prep (Sigma).
Peptide competition (shown above lanes) at 1ug/ml.
This peptide blocking study shows that ab9051 is specific for Histone H4 (mono methyl K20). The activity of the antibody is specifically blocked by the addition of the H4 mono methyl peptide (ab17043).
ChIP - Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab9051 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Immunocytochemistry/ Immunofluorescence - Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
Rabbit polyclonal to Histone H4 mono methyl K20 (1/250).
HeLa cells cultured on glass coverslips were fixed with 4% paraformaldehyde and then stained with ab9051 (green). Total chromatin was visualized using DAPI staining (red).
This image is part of the antibody characterisation at www.cellnucleus.com
Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
Immunocytochemistry/ Immunofluorescence - Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
Rabbit polyclonal to Histone H4 mono methyl K20 (1/250).
HeLa cells cultured on glass coverslips were fixed with 4% paraformaldehyde and then stained with ab9051 (green). Total chromatin was visualized using DAPI staining (red).
Left: merge; center: DAPI; right: Mono K20
This image is part of the antibody characterisation at www.cellnucleus.com
Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
Western blot - Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
All lanes : Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate (ab121)
Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Histone H4 peptide (17-24) - di methyl K20 at 0.5 µg/ml
Lane 8 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 10 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lane 11 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with
Lysates/proteins at 0.5 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Immunocytochemistry/ Immunofluorescence - Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
Rabbit polyclonal to Histone H4 mono methyl K20 (1/250).
10T1 cells cultured on glass coverslips were fixed with 4% paraformaldehyde and then stained with ab9051.
Top left: metaphase cell. Mono methyl K20 (green); DAPI (red)
Top right: interphase cell. Mono methyl K20 (green); DAPI (red)
Lower left: interphase cell. Mono methyl K20 (green); DAPI (blue), TBP (red)
Lower right: interphase cell. Mono methyl K20 (green); DAPI (red)
This image is part of the antibody characterisation at www.cellnucleus.com
Kirk McManus in the lab of Michael Hendzel, Univeristy of Alberta
References for Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051)
This product has been referenced in:
- Ram O et al. Combinatorial patterning of chromatin regulators uncovered by genome-wide location analysis in human cells. Cell 147:1628-39 (2011). CHIPseq; Human.Read more (PubMed: 22196736) »
- Fazzio TG & Panning B Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells. J Cell Biol 188:491-503 (2010).Read more (PubMed: 20176923) »
See all 20 publications for this product
Publishing research using ab9051? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"