Anti-Homer1 antibody (ab88827)
Key features and details
- Mouse polyclonal to Homer1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Homer1 antibody
See all Homer1 primary antibodies -
Description
Mouse polyclonal to Homer1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human Homer1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Rat brain tissue lysate, Homer transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
Sequence similarities
Belongs to the Homer family.
Contains 1 WH1 domain. -
Domain
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
Cellular localization
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
- Unigene: 37500 Rat
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Alternative names
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
Images
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Anti-Homer1 antibody (ab88827) at 1/500 dilution + rat brain tissue lysate at 50 µg
Predicted band size: 39 kDa
Observed band size: 39 kDa -
All lanes : Anti-Homer1 antibody (ab88827) at 1/500 dilution
Lane 1 : Homer transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Datasheets and documents
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Datasheet download
References (1)
ab88827 has been referenced in 1 publication.
- Stepan J et al. Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning. Cell Rep 41:111766 (2022). PubMed: 36476872