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Product Name
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HsGAD1 ChIP probe
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Product type
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DNA RNA and cDNA
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Description
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HsGAD1 ChIP probe
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Tested applications
(see key)
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Real Time PCR
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Application notes
(see key)
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Recommended conditions
Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA.
Quantity provided for 200 reactions. Every 50uL reaction should contain the following:
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| Primer Forward (10µM) | 4.5 µl | | Primer Reverse (10µM) | 4.5 µl | | Probe (5µM) | 2.5 µl | | Taqman mix | x µl (according to the manufacturer’s instructions) | | H2O (Nuclease free) | x µl (to 50µl) |
Amplification:
Stage 1 - 2 min at 50°C - 1 repetition
Stage 2 - 10 mins at 95°C - 1 repetition
Stage 3 - 30 sec at 95°C, 1 min at 60°C – 35-40 repetitions
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Research areas
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Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
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Product overview
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Accession number: NM_000817.The HsGAD1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.
GAD1 (Glutamic acid decarboxylase) is expressed in neurons but silenced in most other cell types. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types GAD1 is enriched with histone modifications associated with silencing (e.g. Histone H3 K9 mono methylation, see image with ab9045). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441).
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Storage buffer
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Constituents: ddH20
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Form
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Liquid
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Concentration
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5 µM
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HsGAD1 ChIP probe is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to GAD1
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HsGAD1 ChIP probe - more information
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