Anti-Hsc70 antibody [1B5] (ab19136)

Overview

• Product nameAnti-Hsc70 antibody [1B5]See all Hsc70 primary antibodies ...
• Description
  Rat monoclonal [1B5] to Hsc70
• Specificityab19136 detects a 73 kDa protein on HeLa heat shocked cell lysate SDS-PAGE immunoblots, corresponding to the molecular weight of Hsc70.
• Tested applicationsIHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB, IP, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Physarum polycephalum
  
• Immunogen

  Full length native Hsc70 protein (Hamster) purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number1B5
• IsotypeIgG2a
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

Applications

Target

• FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the heat shock protein 70 family.
• DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
  ISGylated.
• Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

• Target information above from: UniProt accession P11142 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 395853 Chicken
  • Entrez Gene: 281831 Cow
  • Entrez Gene: 3312 Human
  • Entrez Gene: 15481 Mouse
  • Entrez Gene: 24468 Rat
  • Omim: 600816 Human
  • SwissProt: O73885 Chicken
  • SwissProt: P19120 Cow

  • SwissProt: P11142 Human
  • SwissProt: P63017 Mouse
  • SwissProt: P63018 Rat
  • Unigene: 180414 Human
  • Unigene: 290774 Mouse
  • Unigene: 336743 Mouse
  • Unigene: 351377 Mouse
  • Unigene: 412745 Mouse
  • Unigene: 120392 Rat
  • Unigene: 201298 Rat

  see all
• Alternative names
  • 2410008N15Rik antibody
  • Constitutive heat shock protein 70 antibody
  • Heat shock 70 kDa protein 8 antibody

  • Heat shock 70kD protein 10 antibody
  • Heat shock 70kD protein 8 antibody
  • Heat shock 70kDa protein 8 antibody
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  see all

Anti-Hsc70 antibody [1B5] images

• 

  Immunocytochemistry/ Immunofluorescence - Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous Abreview
  ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.

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• 

  Western blot - Hsc70 antibody [1B5] (ab19136)
  All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml
  
  Lane 1 : G361 human melanoma cells - whole cell lysate (50000 cells/lane)
  Lane 2 : T47D human breast cancer cells - whole cell lysate (50000 cells/lane)
  
  Secondary
  HRP conjugated rabbit anti-rat IgG
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Predicted band size : 73 kDa
  Observed band size : 73 kDa
  
  
  Exposure time : 15 seconds

  This image is courtesy of an anonymous Abreview

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• 

  Western blot - Hsc70 antibody [1B5] (ab19136)
  All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml
  
  Lane 1 : Molecular weight marker
  Lane 2 : Hsc70 (Hsp73) recombinant protein
  Lane 3 : Hsp70 (Hsp72) protein
  Lane 4 : Cell lysates prepared from 3T3 cells
  Lane 5 : Cell lystes prepared from human PC-12 cells
  Lane 6 : Cell lysates prepared from CHO cells
  Lane 7 : Cell lysates prepared from GPC-16 cells
  Lane 8 : Cell lysates prepared from human Hela cells
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 73 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous Abreview
  ab19136 staining Hsc70 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/150) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

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• 

  Flow Cytometry-Anti-Hsc70 antibody [1B5](ab19136)
  Overlay histogram showing HeLa cells stained with ab19136 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19136, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.