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Overview

  • Product nameAnti-Hsc70 antibody [1B5]
    See all Hsc70 primary antibodies
  • Description
    Rat monoclonal [1B5] to Hsc70
  • Specificityab19136 detects a 73 kDa protein on HeLa heat shocked cell lysate SDS-PAGE immunoblots, corresponding to the molecular weight of Hsc70.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Physarum polycephalum
  • Immunogen

    Full length native Hsc70 protein (Hamster) purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number1B5
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.1 µg/ml. Predicted molecular weight: 73 kDa.
IP Use a concentration of 5 µg/ml.
ICC Use a concentration of 5 µg/ml.

Target

  • FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat shock protein family A (Hsp70) member 8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 antibody [1B5] images

  • Western blot - Anti-Hsc70 antibody [1B5] (ab19136)
    Western blot - Anti-Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : G361 human melanoma cells - whole cell lysate (50000 cells/lane)
    Lane 2 : T47D human breast cancer cells - whole cell lysate (50000 cells/lane)

    Secondary
    HRP conjugated rabbit anti-rat IgG
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa


    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [1B5] (ab19136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous Abreview
    ab19136 staining Hsc70 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/150) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Hsc70 antibody [1B5] (ab19136)
    Western blot - Anti-Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous abreview.
    All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HEC59 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-ray HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 22°C.

    See Abreview

  • Western blot - Anti-Hsc70 antibody [1B5] (ab19136)
    Western blot - Anti-Hsc70 antibody [1B5] (ab19136)
    All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Hsc70 (Hsp73) recombinant protein
    Lane 3 : Hsp70 (Hsp72) protein
    Lane 4 : Cell lysates prepared from 3T3 cells
    Lane 5 : Cell lystes prepared from human PC-12 cells
    Lane 6 : Cell lysates prepared from CHO cells
    Lane 7 : Cell lysates prepared from GPC-16 cells
    Lane 8 : Cell lysates prepared from human Hela cells

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 73 kDa
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1B5] (ab19136)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1B5] (ab19136)This image is courtesy of an anonymous Abreview
    ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.

    See Abreview

  • Flow Cytometry - Anti-Hsc70 antibody [1B5] (ab19136)
    Flow Cytometry - Anti-Hsc70 antibody [1B5] (ab19136)
    Overlay histogram showing HeLa cells stained with ab19136 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19136, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Hsc70 antibody [1B5] (ab19136)

This product has been referenced in:
  • Miles AL  et al. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28296633) »
  • Su Hui Teo C  et al. Spatial and Temporal Resolution of Global Protein Synthesis during HSV Infection Using Bioorthogonal Precursors and Click Chemistry. PLoS Pathog 12:e1005927 (2016). Read more (PubMed: 27706239) »

See all 18 Publications for this product

Publishing research using ab19136? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit an abreview for ab19136 Submit a question for ab19136

7 Abreviews or Q&A

10
Votes: Highest First

I am thinking of investigating the cell surface HSC70 expression level in some of cell lines by FACS analysis. Does The FACS data that I can see on the web show that Jurkat cells is expressing HSC70 on the cell surface? I just want to know whether Anti...

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According to the protein database entry http://www.uniprot.org/uniprot/P11142
the protein is located as follows:

Cytoplasm. Melanosome. Nucleus › nucleolus. Note: Localized in cytoplasmic mRNP granules containing untranslated m...

Read More

I am interested in the purchase of two Abcam antibodies:

LAMP2b (cat # ab18529) and HSC70 (ab19136).

However, before purchasing them, we would like to make sure that they work and recognize the proteins in our cellular system (mou...

Read More

Thank you very much for your interest in our products.

We do not offer free or trial sizes of our products simply because we carry over 100,000 items and having multiple sizes would be logistically impossible with our automated freezers. Read More

Western blot abreview for Anti-Hsc70 antibody [1B5]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela, HEC59, HCT116)
Loading amount 30 µg
Specification Hela, HEC59, HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Abcam user community

Verified customer

Submitted Feb 21 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hsc70 antibody [1B5]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast)
Specification Breast
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Sep 13 2010

Western blot abreview for Anti-Hsc70 antibody [1B5]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (melanoma (G361), breast cancer (T47D) cell lines)
Loading amount 50000 cells
Specification melanoma (G361), breast cancer (T47D) cell lines
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5%
Read More
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Abcam user community

Verified customer

Submitted Oct 09 2006

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsc70 antibody [1B5]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 30 minute(s) · Concentration: n/a
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Abcam user community

Verified customer

Submitted Sep 29 2006

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsc70 antibody [1B5]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal neurons)
Specification cultured hippocampal neurons
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
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Dr. Vladimir Sytnyk

Verified customer

Submitted Aug 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"