Anti-Hsc70 antibody [EP1531Y] (ab51052)

Overview

• Product nameAnti-Hsc70 antibody [EP1531Y]See all Hsc70 primary antibodies ...
• Description
  Rabbit monoclonal [EP1531Y] to Hsc70
• Tested applicationsFlow Cyt, IP, WB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C terminus of human Hsc70.

• Positive controlWB: HeLa cell lysate IHC-P: Human breast carcinoma tissue ICC/IF: HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1531Y
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

Applications

Target

• FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the heat shock protein 70 family.
• DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
  ISGylated.
• Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P11142 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3312 Human
  • Entrez Gene: 15481 Mouse
  • Entrez Gene: 24468 Rat
  • Omim: 600816 Human
  • SwissProt: P11142 Human
  • SwissProt: P63017 Mouse
  • SwissProt: P63018 Rat
  • Unigene: 180414 Human

  • Unigene: 290774 Mouse
  • Unigene: 336743 Mouse
  • Unigene: 351377 Mouse
  • Unigene: 412745 Mouse
  • Unigene: 120392 Rat
  • Unigene: 201298 Rat

  see all

• Alternative names
  2410008N15Rik antibodyConstitutive heat shock protein 70 antibodyHeat shock 70 kDa protein 8 antibody

  Heat shock 70kD protein 10 antibodyHeat shock 70kD protein 8 antibodyHeat shock 70kDa protein 8 antibodyHeat shock cognate 71 kDa protein antibodyHeat shock cognate protein 54 antibodyHeat shock cognate protein 71 kDa antibodyHeat shock protein 8 antibodyHeat shock protein A8 antibodyHeat-shock70-KD protein 10, formerly antibodyHSC54 antibodyHSC71 antibodyHsc73 antibodyHSP71 antibodyHSP73 antibodyHSP7C_HUMAN antibodyHSPA10 antibodyHSPA8 antibodyLAP1 antibodyLipopolysaccharide associated protein 1 antibodyLPS associated protein 1 antibodyLPS associated protein antibodyMGC102007 antibodyMGC106514 antibodyMGC114311 antibodyMGC118485 antibodyMGC131511 antibodyMGC29929 antibodyN-myristoyltransferase inhibitor protein 71 antibodyNIP71 antibody

  see all

Anti-Hsc70 antibody [EP1531Y] images

• 

  Western blot - Hsc70 antibody [EP1531Y] (ab51052)
  Anti-Hsc70 antibody [EP1531Y] (ab51052) at 1/500 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  Goat anti-Rabbit HRP labeled at 1/2000 dilution
  
  Predicted band size : 71 kDa
  Observed band size : 71 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Hsc70 antibody [EP1531Y] (ab51052)
  Ab51052 (1/250) staining Hsc70 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Hsc70 antibody [EP1531Y] (ab51052)
  Immunofluorescent staining of HeLa cells using ab51052 (1/100).