Overview

  • Product nameAnti-Hsc70 antibodySee all Hsc70 primary antibodies ...
  • Description
    Rabbit polyclonal to Hsc70
  • SpecificityThe antibody detects a 73 kDa protein, corresponding to the apparent molecular weight of Hsc70 on SDS-PAGE immunoblots. No reactivity with inducible Hsp70 is detected.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide:

    EKLQGKINDEDKQKI

    , corresponding to amino acids 555-570 of Human Hsc70

  • General notes


    The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Primary antibody notes The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate 71 kDa protein. antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock cognate protein 71-kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 antibody images

  • Ab1427 staining human normal testis tissue. Staining is localised to cytoplasmic and nuclear compartments.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-Hsc70 antibody (ab1427) at 1/2500 dilution

    Lane 1 : U2OS cell lysate
    Lane 2 : HeLa cell lysate

    Secondary
    HRP-conjugated Goat polyclonal to Rabbit at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Hsc70 antibody (ab1427)

This product has been referenced in:
  • Dermentzaki G  et al. Loss of ß-glucocerebrosidase activity does not affect alpha-synuclein levels or lysosomal function in neuronal cells. PLoS One 8:e60674 (2013). WB ; Human . Read more (PubMed: 23580063) »
  • Acuña Sanhueza GA  et al. Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells. BMC Cancer 12:72 (2012). WB ; Human . Read more (PubMed: 22339894) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Sheep Serum (Seminal Plasma)
Specification Seminal Plasma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Guillaume TSIKIS

Verified customer

Submitted Jul 15 2013

Application Western blot
Sample Human Cell lysate - whole cell (U2OS and HeLa cells)
Loading amount 30 µg
Specification U2OS and HeLa cells
Gel Running Conditions Reduced Denaturing (12% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 18 2011

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You are largely following an approach that I would recommend; using 3T3 mo...

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Dear Dr. Lloyd, Thank you for your enquiry. Regarding ab1427 (Hsc70 antibody), this product has been Protein A/G purified.

Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with ab1427. We have not received any other complaints regarding this antibody and it may be that the vial you received was damaged. I would be happy to send you a...

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Thank you for your enquiry regarding ab1427. Synthetic peptide surrounding amino acid 559 of human Hsc70 was used to raise this antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"