Overview

  • Product name
  • Description
    Goat polyclonal to Hsc70
  • Specificity
    This antibody is expected to recognise isoform 1 (NP_006588.1) only.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Dog, Orangutan
  • Immunogen

    Synthetic peptide:

    C-EKLQGKINDEDKQK

    , corresponding to internal sequence amino acids 556-569 of Human Hsc70

  • Positive control
    • A431 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:4,000.
    WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the heat shock protein 70 family.
    • Domain
      The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • Cellular localization
      Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2410008N15Rik antibody
      • Constitutive heat shock protein 70 antibody
      • Epididymis luminal protein 33 antibody
      • Epididymis secretory sperm binding protein Li 72p antibody
      • Heat shock 70 kDa protein 8 antibody
      • Heat shock 70kD protein 10 antibody
      • Heat shock 70kD protein 8 antibody
      • Heat shock 70kDa protein 8 antibody
      • Heat shock cognate 71 kDa protein antibody
      • Heat shock cognate protein 54 antibody
      • Heat shock cognate protein 71 kDa antibody
      • Heat shock protein 8 antibody
      • Heat shock protein A8 antibody
      • Heat shock protein family A (Hsp70) member 8 antibody
      • Heat-shock70-KD protein 10, formerly antibody
      • HEL 33 antibody
      • HEL S 72p antibody
      • HSC54 antibody
      • HSC71 antibody
      • Hsc73 antibody
      • HSP71 antibody
      • HSP73 antibody
      • HSP7C_HUMAN antibody
      • HSPA10 antibody
      • HSPA8 antibody
      • LAP1 antibody
      • Lipopolysaccharide associated protein 1 antibody
      • LPS associated protein 1 antibody
      • LPS associated protein antibody
      • MGC102007 antibody
      • MGC106514 antibody
      • MGC114311 antibody
      • MGC118485 antibody
      • MGC131511 antibody
      • MGC29929 antibody
      • N-myristoyltransferase inhibitor protein 71 antibody
      • NIP71 antibody
      see all

    Images

    References

    ab52057 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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