Hsp 27 Human ELISA Kit (ab108862)
- Product nameHsp 27 Human ELISA KitSee all Heat Shock Protein 27 (HSP27) kits ...
- Tests1 x 96 well plate
- Sample typeMilk, Serum, Plasma, Cell culture extracts, Tissue
- Assay typeSandwich
- Sensitivity= 0.3 ng/ml
- Range0.313 ng/ml - 80 ng/ml
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
Heat shock proteins are molecular chaperones that have an ability to protect proteins from damage induced by environmental factors such as free radicals, heat, ischaemia and toxins, allowing denatured proteins to adopt their native configuration. Heat shock protein 27 (Hsp 27) is a member of the small Hsp (sHsp) family of proteins, and has a molecular weight of approximately 27 kDa. In addition to its role as a chaperone, it has also been reported to have many additional functions. These include effects on the apoptotic pathway, cell movement and embryogenesis.
It is suggested that Hsp 27 may play a key role in resistance to doxorubicin-induced cardiac dysfunction, and lower lymphocyte Hsp 27 levels might be associated with an increased risk of lung cancer. Hsp 27 expression is enhanced in target tissues of diabetic microvascular complications, and changes in circulating serum Hsp 27 levels (sHSP 27) have been reported in patients with macrovascular disease.
ab108862 Hsp 27 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Hsp 27 in plasma, serum, milk, tissue extract and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Human Hsp 27 in less than 5 hours. A polyclonal antibody specific for Human Hsp 27 has been pre-coated onto a 96-well microplate with removable strips. Hsp 27 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Hsp 27, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Hsp 27 Antibody (100x): 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Hsp 27 Standard 1 unit Human Hsp27 Microplate: 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- HSPB128 kDa heat shock proteinCMT2F
- estrogen regulated 24 kDa proteinEstrogen-regulated 24 kDa proteinHeat shock 27 kDa proteinheat shock 27kDa protein 1heat shock protein beta 1Heat shock protein beta-1HMN2BHS.76067HSP 27Hsp25HSP27HSP28HspB1SRP27stress responsive protein 27Stress-responsive protein 27
Our Abpromise guarantee covers the use of ab108862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Hsp 27 Human ELISA Kit (ab108862)
ab108862 has not yet been referenced specifically in any publications.