Anti-Hsp104 antibody (ab69549)
Key features and details
- Rabbit polyclonal to Hsp104
- Suitable for: WB, IP
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Hsp104 antibody
See all Hsp104 primary antibodies -
Description
Rabbit polyclonal to Hsp104 -
Host species
Rabbit -
Specificity
ab69549 detects an ~104 kDa protein, corresponding to the apparent molecular mass of Hsp104 on SDS-PAGE immunoblots, in samples from yeast (S. cerevisiae) origins. This antibody also detects an ~50 kDa band in yeast samples on immunoblot analysis. No reactivity is detected in immunoblot analysis with human, mouse, rat, monkey, and hamster proteins. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Synthetic peptide:
DDDNEDSMEIDDDLD
conjugated to KLH, corresponding to amino acids 894-908 of Saccharomyces cerevisiae Hsp104 -
Positive control
- S. cerevisiae cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 104, 50 kDa (predicted molecular weight: 102 kDa).
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IP |
1/100.
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Notes |
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WB
1/5000. Detects a band of approximately 104, 50 kDa (predicted molecular weight: 102 kDa). |
IP
1/100. |
Target
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Relevance
Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 is vital to protect yeast cells against high temperature and high concentration of ethanol. It is not required for normal growth. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 850633 Saccharomyces cerevisiae
- SwissProt: P31539 Saccharomyces cerevisiae
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Alternative names
- L0948 antibody
- Heat shock protein 104 antibody
- Hsp 104 antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab69549 has been referenced in 13 publications.
- Andersson S et al. Genome-wide imaging screen uncovers molecular determinants of arsenite-induced protein aggregation and toxicity. J Cell Sci 134:N/A (2021). PubMed: 34085697
- Huang YW et al. Mutable yeast prion variants are stabilized by a defective Hsp104 chaperone. Mol Microbiol 115:774-788 (2021). PubMed: 33190361
- Wang Y et al. Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer. Cell Chem Biol 28:1283-1297.e8 (2021). PubMed: 33667410
- Schneider KL et al. Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research. Sci Rep 11:12819 (2021). PubMed: 34140587
- Cereghetti G et al. Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly. Nat Cell Biol 23:1085-1094 (2021). PubMed: 34616026