Anti-Hsp27 antibody [8A7] (ab79868)
Key features and details
- Mouse monoclonal [8A7] to Hsp27
- Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp27 antibody [8A7]
See all Hsp27 primary antibodies -
Description
Mouse monoclonal [8A7] to Hsp27 -
Host species
Mouse -
Specificity
ab79868 recognizes Hsp25 and Hsp27, and cross reacts with alpha B crystallin. -
Tested applications
Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Human Hsp27 peptide
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8A7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100.
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ICC/IF |
1/200.
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WB |
1/5000. Predicted molecular weight: 23 kDa.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. |
ICC/IF
1/200. |
WB
1/5000. Predicted molecular weight: 23 kDa. |
Target
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Function
Involved in stress resistance and actin organization. -
Tissue specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. -
Involvement in disease
Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Post-translational
modificationsPhosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 3315 Human
- Entrez Gene: 15507 Mouse
- Entrez Gene: 24471 Rat
- Omim: 602195 Human
- SwissProt: P04792 Human
- SwissProt: P14602 Mouse
- SwissProt: P42930 Rat
- Unigene: 520973 Human
see all -
Alternative names
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
see all
Images
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Immunohistochemistry analysis of Mouse backskin tissue using Mouse Anti-Hsp27 Monoclonal Antibody, at 1:100 dilution for 1 hour at RT. FITC Goat Anti-Mouse (green) was used as a Secondary Antibody at 1:50 dilutionfor 1 hour. Localization: Epidermis.
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Anti-Hsp27 antibody [8A7] (ab79868) at 1/1000 dilution + Rat lung tissue lysate
Predicted band size: 23 kDa -
ab79868 at 1/100,000 dilution staining Hsp27 in human colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining.
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ab79868 (1/100) staining Hsp27 in Mouse epidermis tissue sections by IF (Iimmunofluorescence). Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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ab79868 staining Hsp27 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).
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Overlay histogram showing HeLa cells stained with ab79868 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79868, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab79868 has been referenced in 8 publications.
- Zheng W et al. Effects of transport stress on pathological injury and main heat shock protein expression in the respiratory system of goats. J Anim Physiol Anim Nutr (Berl) 105:1-13 (2021). PubMed: 32744367
- Ma D et al. Elucidating the involvement of apoptosis in postmortem proteolysis in porcine muscles from two production cycles using metabolomics approach. Sci Rep 11:3465 (2021). PubMed: 33568769
- Hu W et al. Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach. BMC Vet Res 16:347 (2020). PubMed: 32962700
- Yang X et al. The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens). PLoS One 12:e0176451 (2017). WB, IHC ; Other species . PubMed: 28463974
- Peterson CW et al. Heat-shock protein expression in canine corneal wound healing. Vet Ophthalmol 19:262-6 (2016). PubMed: 26302381
- Chen A et al. Lysine deacetylase inhibition promotes relaxation of arterial tone and C-terminal acetylation of HSPB6 (Hsp20) in vascular smooth muscle cells. Physiol Rep 1:e00127 (2013). PubMed: 24400135
- Jia Y et al. Identification and characterization of hic-5/ARA55 as an hsp27 binding protein. J Biol Chem 276:39911-8 (2001). IP . PubMed: 11546764
- Welsh MJ et al. Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat. Biol Reprod 55:141-51 (1996). WB, ICC/IF . PubMed: 8793069