Anti-Hsp40 antibody [2A7.H6] (ab74442)
Key features and details
- Mouse monoclonal [2A7.H6] to Hsp40
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-Hsp40 antibody [2A7.H6]
See all Hsp40 primary antibodies -
Description
Mouse monoclonal [2A7.H6] to Hsp40 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae
Does not react with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Saccharomyces cerevisiae Hsp40.
Database link: P25491 -
Positive control
- WB: Yeast recombinant cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A7.H6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/2000. Predicted molecular weight: 40 kDa. |
Target
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Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
Sequence similarities
Contains 1 J domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
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Alternative names
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab74442 has been referenced in 2 publications.
- Lian HY et al. Generation and propagation of yeast prion [URE3] are elevated under electromagnetic field. Cell Stress Chaperones 23:581-594 (2018). PubMed: 29214607
- Hamdan N et al. ER stress causes widespread protein aggregation and prion formation. J Cell Biol 216:2295-2304 (2017). PubMed: 28630146