Anti-Hsp60 antibody [LK-2] (ab59458)
Key features and details
- Mouse monoclonal [LK-2] to Hsp60
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp60 antibody [LK-2]
See all Hsp60 primary antibodies -
Description
Mouse monoclonal [LK-2] to Hsp60 -
Host species
Mouse -
Specificity
Detects ~60kDa.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Human Hsp60 produced through recombinant DNA methods in E.coli
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Positive control
- HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
LK-2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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WB | (1) |
1/40000. Predicted molecular weight: 60 kDa.
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Notes |
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IHC-P
1/100. |
WB
1/40000. Predicted molecular weight: 60 kDa. |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- Unigene: 595053 Human
- Unigene: 727543 Human
- Unigene: 1777 Mouse
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Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.
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Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 60 kDa -
Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution
Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
Lane 4 : Ladder
Secondary
Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 25 seconds
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ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.
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Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab59458 has been referenced in 6 publications.
- Jiang X et al. Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants. Commun Biol 4:4 (2021). PubMed: 33398015
- Senger F et al. Spatial integration of mechanical forces by a-actinin establishes actin network symmetry. J Cell Sci 132:N/A (2019). PubMed: 31615968
- Farrugia G et al. Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells. Sci Rep 9:6152 (2019). PubMed: 30992471
- Schultz MJ et al. Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures. J Virol 91:N/A (2017). PubMed: 28446677
- Mion F et al. Mast cells control the expansion and differentiation of IL-10-competent B cells. J Immunol 193:4568-79 (2014). PubMed: 25267976
- Bivi N et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). PubMed: 19226166