Anti-Hsp70 antibody [3A3] (ab74084)

Overview

  • Product nameAnti-Hsp70 antibody [3A3]See all Hsp70 primary antibodies ...
  • Description
    Mouse monoclonal [3A3] to Hsp70
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Fish
    Predicted to work with: Amphibians
  • Immunogen

    Recombinant full length protein (Human) expressed in E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab74084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/500.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificityHSPA1B is testis-specific.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P08107 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DAQB 147D11.1 001 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • Heat shock protein 70 antibody
    • HSP70 1 antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    • XXbac BCX40G17.3 001 antibody
    see all

Anti-Hsp70 antibody [3A3] images

  • Anti-Hsp70 antibody [3A3] (ab74084) at 1/1000 dilution + rat tissue lysate

    Secondary
    HRP-conjugated goat anti mouse

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Hsp70 antibody [3A3] (ab74084)

ab74084 has not yet been referenced specifically in any publications.

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I am now back in the office and have reviewed this case for you. Regrettably, we have no other Hsp70 antibodies that have been tested in Drosophila or that have specifically been tested andguaranteed not to reac...

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