Overview

  • Product nameAnti-Hsp70 antibody [5A5]See all Hsp70 primary antibodies ...
  • Description
    Mouse monoclonal [5A5] to Hsp70
  • SpecificityThe antibody will recognize both Hsp70 and Hsc70 in mouse tissues.
  • Tested applicationsIHC-P, IHC-Fr, ICC/IF, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Fish
    Predicted to work with: Bird, Amphibians
  • Immunogen

    Recombinant full length protein (Human) expressed in E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab74072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
ICC/IF 1/500.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificityHSPA1B is testis-specific.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P08107 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DAQB 147D11.1 001 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • Heat shock protein 70 antibody
    • HSP70 1 antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    • XXbac BCX40G17.3 001 antibody
    see all

Anti-Hsp70 antibody [5A5] images

  • Anti-Hsp70 antibody [5A5] (ab74072) at 1/1000 dilution + Rat lysates

    Secondary
    HRP-conjugated goat anti mouse IgG

    Predicted band size : 70 kDa

References for Anti-Hsp70 antibody [5A5] (ab74072)

ab74072 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"