Overview

  • Product nameAnti-Hsp70 antibody [EP1007Y]
    See all Hsp70 primary antibodies
  • Description
    Rabbit monoclonal [EP1007Y] to Hsp70
  • Tested applicationsFlow Cyt, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human Hsp70.

  • Positive control
    • HeLa cell lysate,human prostate carcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/1000.
IP 1/50.
WB 1/20000. Detects a band of approximately 85 kDa.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
    • Tissue specificityHSPA1B is testis-specific.
    • Sequence similaritiesBelongs to the heat shock protein 70 family.
    • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links
    • Alternative names
      • DnaK type molecular chaperone HSP70 1 antibody
      • Epididymis secretory protein Li 103 antibody
      • FLJ54303 antibody
      • FLJ54370 antibody
      • FLJ54392 antibody
      • FLJ54408 antibody
      • FLJ75127 antibody
      • Heat shock 70 kDa protein 1 antibody
      • Heat shock 70 kDa protein 1/2 antibody
      • Heat shock 70 kDa protein 1A/1B antibody
      • Heat shock 70kDa protein 1A antibody
      • Heat shock 70kDa protein 1B antibody
      • Heat shock induced protein antibody
      • HEL S 103 antibody
      • HSP70 1 antibody
      • HSP70 1B antibody
      • HSP70 2 antibody
      • HSP70-1/HSP70-2 antibody
      • HSP70-1A antibody
      • HSP70.1 antibody
      • HSP70.1/HSP70.2 antibody
      • HSP70I antibody
      • HSP71_HUMAN antibody
      • HSP72 antibody
      • HSPA1 antibody
      • HSPA1A antibody
      • HSPA1B antibody
      see all

    Anti-Hsp70 antibody [EP1007Y] images

    • Overlay histogram showing Jurkat cells stained with ab45133 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45133, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-Hsp70 antibody [EP1007Y] (ab45133) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Ab45133 at a 1/100 dilution staining human Hsp70 in human prostate carcinoma by immunohistochemistry using paraffin embedded tissue.Ab45133 at a 1/100 dilution staining human Hsp70 in human prostate carcinoma by immunohistochemistry using paraffin embedded tissue.


    • Exposure time : 2 minutes

    References for Anti-Hsp70 antibody [EP1007Y] (ab45133)

    This product has been referenced in:
    • Leu JI  et al. A small molecule inhibitor of inducible heat shock protein 70. Mol Cell 36:15-27 (2009). WB, IP ; Human . Read more (PubMed: 19818706) »
    • Zhang C  et al. Sodium butyrate upregulates expression of NKG2D ligand MICA/B in HeLa and HepG2 cell lines and increases their susceptibility to NK lysis. Cancer Immunol Immunother 58:1275-85 (2009). Read more (PubMed: 19139882) »

    See all 2 Publications for this product

    Product Wall

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    Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

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