Overview

  • Product nameAnti-Hsp70 antibodySee all Hsp70 primary antibodies ...
  • Description
    Rabbit polyclonal to Hsp70
  • SpecificityThis antibody reacts with the two major human HSP 70s (HSP 72 and HSP 73). It stains epithelial cells in many different tissues e.g. tonsil, stomach, small and large intestine, bile ducts, ovary and prostate.
  • Tested applicationsIHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified heat shock protein (Dnak) from E. coli.

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab2412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75 - 1/250. Use an ABC method and incubate for 30 minutes at room temperature.
IHC-Fr 1/75 - 1/250. Use an ABC method and incubate for 30 minutes at room temperature.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 70 kDa).
  • Application notesIs unsuitable for ELISA.
  • Target

    • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
    • Tissue specificityHSPA1B is testis-specific.
    • Sequence similaritiesBelongs to the heat shock protein 70 family.
    • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAQB 147D11.1 001 antibody
      • FLJ54303 antibody
      • FLJ54370 antibody
      • FLJ54392 antibody
      • FLJ54408 antibody
      • FLJ75127 antibody
      • Heat shock 70 kDa protein 1 antibody
      • Heat shock 70 kDa protein 1/2 antibody
      • Heat shock 70 kDa protein 1A/1B antibody
      • heat shock 70kDa protein 1A antibody
      • Heat shock 70kDa protein 1B antibody
      • Heat shock induced protein antibody
      • heat shock protein 70 antibody
      • HSP70 1 antibody
      • HSP70 2 antibody
      • HSP70-1/HSP70-2 antibody
      • HSP70-1A antibody
      • HSP70.1 antibody
      • HSP70.1/HSP70.2 antibody
      • HSP70I antibody
      • HSP71_HUMAN antibody
      • HSP72 antibody
      • HSPA1 antibody
      • HSPA1A antibody
      • HSPA1B antibody
      • XXbac BCX40G17.3 001 antibody
      see all

    Anti-Hsp70 antibody images

    • ab2412 staining Hsp70 in human breast carcinoma cells
    • All lanes : Anti-Hsp70 antibody (ab2412) at 1 µg/ml

      Lane 1 : Heart (Rat) Tissue Lysate
      Lane 2 : Spleen (Rat) Tissue Lysate
      Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 70 kDa
      Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining Hsp70 with ab2412.

    References for Anti-Hsp70 antibody (ab2412)

    ab2412 has not yet been referenced specifically in any publications.

    Product Wall

    I received this morning the following information from the source of ab2412: "we have not seen any drop in the quality of ab2412 in IHC, neither have we heard any complaints for lot # E676. We have totally exhausted lot # E392 and in fact have only o...

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    I received feedback from the laboratory that tested ab2412 in immunohistochemistry who confirmed there are no quality concerns with lot 165620 following those tests. As the antibody has not been tested in ELISA we cannot tell whether another lot would...

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    Thank you for contacting us for technical support regarding ab2412. To our knowledge this antibody had not been tested in ELISA so it is good to know that you have had good results with this antibody in the past. Currently the ELISA application is no...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"