• Product name
  • Description
    Rabbit polyclonal to Hsp70
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Hsp70.

  • Positive control
    • Hela cells. Breast cancer.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab31010 is an affinity purified IgG.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab31010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/100 for 30 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM
    citrate buffer, pH 6.0 for 10 min and then cool at RT for 20 min.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target


    • Hsp70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Hsp70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31010.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 70kDa; Hsp70

    • Tissue: Human breast cancer (formalin fixed/paraffin).
      Staining: ab31010 at 1/100 dilution for 30 minutes at room temperature.
    • Anti-Hsp70 antibody (ab31010) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 70 kDa
      Observed band size : 72 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 76 kDa (possible post-translational modification).
    • Anti-Hsp70 antibody (ab31010) at 1/25 dilution + 293 cell lysate

      Predicted band size : 70 kDa
      Observed band size : 70 kDa


    This product has been referenced in:
    • Ulbrich F  et al. The Carbon monoxide releasing molecule ALF-186 mediates anti-inflammatory and neuroprotective effects via the soluble guanylate cyclase ß1 in rats' retinal ganglion cells after ischemia and reperfusion injury. J Neuroinflammation 14:130 (2017). WB ; Rat . Read more (PubMed: 28655348) »
    • Banerjee S  et al. Minnelide reduces tumor burden in preclinical models of osteosarcoma. Cancer Lett 335:412-20 (2013). Read more (PubMed: 23499892) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 09 2016

    Thank you for calling Abcam earlier today.

    I have talked to the lab and ab31010 was raised against a synthetic peptide corresponding to The IHC and WB data both show results appropriate for HSP-70-1/2, thus we would expect that you should see...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Wir haben keinen Antikörper gegen HSP70 oder 90 im Katalog, der schon für Aspergillus terreus getestet ist.

    Ich habe die Immunogene unserer ...

    Read More

    Thank you for your enquiry. The following reference reports HSP70 in the nuclei of cells at the onset of caspase activation: Nature. 2007 Jan 4;445(7123):102-5. Epub 2006 Dec 10. PMID: 17167422 and this reference describes the subcellular l...

    Read More


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