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Overview

  • Product nameAnti-Hsp70 antibody
    See all Hsp70 primary antibodies
  • Description
    Mouse monoclonal to Hsp70
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Pig, Opossum, Orangutan
  • Immunogen

    Recombinant fragment with tag: VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL Y, corresponding to amino acids 531-612 of Human Hsp70

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab61097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 70 kDa).
IHC-P Use a concentration of 3 µg/ml.
ELISA Use at an assay dependent concentration.

Target

Anti-Hsp70 antibody images

  • Anti-Hsp70 antibody (ab61097) at 1 µg/ml + Hela nuclear extract at 25 µg

    Predicted band size : 70 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
  • ab61097 at 3 ug/ml staining HSPA1B in human esophagus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

References for Anti-Hsp70 antibody (ab61097)

ab61097 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"