Overview

  • Product nameAnti-Hsp70 antibodySee all Hsp70 primary antibodies ...
  • Description
    Rabbit polyclonal to Hsp70
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Caenorhabditis elegans, Zebrafish
    Predicted to work with: Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Pig, African Green Monkey
  • Immunogen

    A region within synthetic peptide: SLFEGIDFYT SITRARFEEL CSDLFRSTLE PVEKALRDAK LDKAQIHDLV, corresponding to amino acids 286-335 of Human Hsp70

  • Positive control
    • Liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

Target

Anti-Hsp70 antibody images

  • Anti-Hsp70 antibody (ab66262) at 1.25 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-Hsp70 antibody (ab66262)

ab66262 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Lung)
Loading amount 20 µg
Specification Lung
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Dec 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"