Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp70
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 308-579 of Human Hsp70 (NP_005336).

  • Positive control
    • A431 and Raji whole cell lysates; A431 cells; 293T, H1299, HeLa, HepG2, and MOLT4 cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab97699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificity
    HSPA1B is testis-specific.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Images

  • All lanes : Anti-Hsp70 antibody (ab97699) at 1/2000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate
    Lane 3 : Hep G2 Whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 70 kDa
  • ab97699, at 1/200 dilution, staining Hsp70 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.

References

ab97699 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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