Anti-Hsp90 alpha antibody, prediluted (ab54265)
Overview
- Product nameAnti-Hsp90 alpha antibody, predilutedSee all Hsp90 alpha primary antibodies ...
- DescriptionRabbit polyclonal to Hsp90 alpha, prediluted
- Tested applicationsIHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Hamster, Cow, Pig, Cynomolgus Monkey, Rhesus monkey - Immunogen
Synthetic peptide: PEETQTQDQPM, corresponding to N terminal amino acids 2-12 of Mouse Hsp90 alpha (Hsp86).
- Positive controlMAD109 cells or breast carcinoma
- General notesPrediluted antibody which is ready-to-use for staining of formalin-fixed, paraffin-embedded tissues.
Properties
- FormPrediluted
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab54265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P |
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
The optimal dilution for a specific application should be determined by the investigator.
Target
- FunctionMolecular chaperone. Has ATPase activity.
- Sequence similaritiesBelongs to the heat shock protein 90 family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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Database links
- Entrez Gene: 281832 Cow
- Entrez Gene: 3320 Human
- Entrez Gene: 15519 Mouse
- Entrez Gene: 397028 Pig
- Entrez Gene: 299331 Rat
- Omim: 140571 Human
- SwissProt: Q76LV2 Cow
- SwissProt: P07900 Human
- SwissProt: P07901 Mouse
- SwissProt: O02705 Pig
- SwissProt: P30946 Rabbit
- SwissProt: P82995 Rat
- Unigene: 525600 Human
- Unigene: 700831 Human
- Unigene: 1843 Mouse
- Unigene: 341186 Mouse
- Unigene: 119867 Rat
see all
Target information above from: UniProt accession
P07900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Heat shock 86 kDa antibodyHeat shock 90kDa protein 1 alpha antibodyHeat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
- Heat shock protein HSP 90-alpha antibodyHS90A_HUMAN antibodyHSP 86 antibodyHSP 86 antibodyHSP86 antibodyHsp89 antibodyHSP89A antibodyHSP90A antibodyHSP90AA1 antibodyHSP90ALPHA antibodyHSP90N antibodyHSPC1 antibodyHSPCA antibodyHSPCAL1 antibodyHSPCAL4 antibodyHSPN antibodyLAP2 antibodyRenal carcinoma antigen NY-REN-38 antibody
see all
Anti-Hsp90 alpha antibody, prediluted images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp90 alpha antibody, prediluted (ab54265)Human breast carcinoma stained with Hsp90 alpha antibody (ab54265)
References for Anti-Hsp90 alpha antibody, prediluted (ab54265)
This product has been referenced in:
- Huang SK et al. LC/MS-based quantitative proteomic analysis of paraffin-embedded archival melanomas reveals potential proteomic biomarkers associated with metastasis. PLoS One 4:e4430 (2009). IHC-P ; Human . Read more (PubMed: 19221597) »
- Ramírez V et al. Radicicol, a heat shock protein 90 inhibitor, reduces glomerular filtration rate. Am J Physiol Renal Physiol 295:F1044-51 (2008). Read more (PubMed: 18667483) »
