Overview

  • Product nameAnti-Hsp90 antibody
    See all Hsp90 primary antibodies
  • Description
    Rabbit polyclonal to Hsp90
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Fruit fly (Drosophila melanogaster), Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Hsp90 aa 289-300.
    Sequence:

    ELNKTKPIWTRN

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab34909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/5000 - 1/25000.

Target

Anti-Hsp90 antibody images

  • All lanes : Anti-Hsp90 antibody (ab34909) at 1/500 dilution

    Lane 1 : Cos7 cells transfected with mutant K294R Hsp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 2 : Cos7 cells transfected with mutant K294R Hsp90, mock treated with DMSO only
    Lane 3 : Cos7 cells transfected wild type Hp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 4 : Cos7 cells transfected wild type Hp90, mock treated with DMSO only
    Lane 5 : Cos 7 cells transfected with empty vector, treated with Trichostatin A (an HDAC inhibitor)
    Lane 6 : Cos 7 cells transfected with empty vector, mock treated with DMSO only


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

References for Anti-Hsp90 antibody (ab34909)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 1e+007 cells
Gel Running Conditions Reduced Denaturing (4-20% gradient gels)
Sample Candida albicans Cell lysate - whole cell (cells grown in liquid YPD)
Specification cells grown in liquid YPD
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Jan 26 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Tissue sections (Eye imaginal disc)
Specification Eye imaginal disc
Fixative Paraformaldehyde
Permeabilization Yes - PBS 0.1% triton
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Abcam user community

Verified customer

Submitted Oct 13 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (n2a cell)
Loading amount 20 µg
Specification n2a cell
Gel Running Conditions Reduced Denaturing (4-12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Feb 27 2008

Thank you for your interesting enquiry. I have looked at the details for all the antibodies we have raised against Hsp90. There is another one available, rabbit polyclonal ab13494 that will be more suitable for your application. It has been tested in E...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"