Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp90
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Hsp90 aa 289-300.
    Sequence:

    ELNKTKPIWTRN

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/5000 - 1/25000.

Target

Anti-Hsp90 antibody images

  • All lanes : Anti-Hsp90 antibody (ab34909) at 1/500 dilution

    Lane 1 : Cos7 cells transfected with mutant K294R Hsp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 2 : Cos7 cells transfected with mutant K294R Hsp90, mock treated with DMSO only
    Lane 3 : Cos7 cells transfected wild type Hp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 4 : Cos7 cells transfected wild type Hp90, mock treated with DMSO only
    Lane 5 : Cos 7 cells transfected with empty vector, treated with Trichostatin A (an HDAC inhibitor)
    Lane 6 : Cos 7 cells transfected with empty vector, mock treated with DMSO only


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

References for Anti-Hsp90 antibody (ab34909)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
1e+007 cells
Gel Running Conditions
Reduced Denaturing (4-20% gradient gels)
Sample
Candida albicans Cell lysate - whole cell (cells grown in liquid YPD)
Specification
cells grown in liquid YPD
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Jan 26 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Tissue sections (Eye imaginal disc)
Specification
Eye imaginal disc
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0.1% triton
Username

Abcam user community

Verified customer

Submitted Oct 13 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (n2a cell)
Loading amount
20 µg
Specification
n2a cell
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 27 2008

Thank you for your interesting enquiry. I have looked at the details for all the antibodies we have raised against Hsp90. There is another one available, rabbit polyclonal ab13494 that will be more suitable for your application. It has been tested in E...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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