Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab34909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/5000 - 1/25000.

Target

  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • Post-translational
    modifications
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6S182 antibody
    • FLJ26984 antibody
    • FLJ31884 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP84 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • Hsp90 antibody
    • HSP90 Beta antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPCB antibody
    • HSPN antibody
    • LAP2 antibody
    • Lipopolysaccharide associated protein2 antibody
    • LPS associated protein 2 antibody
    • NY REN 38 antigen antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 antibody images

  • All lanes : Anti-Hsp90 antibody (ab34909) at 1/500 dilution

    Lane 1 : Cos7 cells transfected with mutant K294R Hsp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 2 : Cos7 cells transfected with mutant K294R Hsp90, mock treated with DMSO only
    Lane 3 : Cos7 cells transfected wild type Hp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 4 : Cos7 cells transfected wild type Hp90, mock treated with DMSO only
    Lane 5 : Cos 7 cells transfected with empty vector, treated with Trichostatin A (an HDAC inhibitor)
    Lane 6 : Cos 7 cells transfected with empty vector, mock treated with DMSO only


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

References for Anti-Hsp90 antibody (ab34909)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Tissue sections (Eye imaginal disc)
Specification Eye imaginal disc
Fixative Paraformaldehyde
Permeabilization Yes - PBS 0.1% triton
Username

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Verified customer

Submitted Oct 13 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (n2a cell)
Loading amount 20 µg
Specification n2a cell
Gel Running Conditions Reduced Denaturing (4-12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 27 2008

Thank you for your interesting enquiry. I have looked at the details for all the antibodies we have raised against Hsp90. There is another one available, rabbit polyclonal ab13494 that will be more suitable for your application. It has been tested in E...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"