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Anti-Hsp90 beta antibody [E296] (ab32568)

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Overview

Product name

Anti-Hsp90 beta antibody [E296]
See all Hsp90 beta products (18) ...

Description

Rabbit monoclonal [E296] to Hsp90 beta

Tested applications

WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues in N-terminus of human Hsp90 beta.

Positive control

Hela cell lysate and urinary bladder carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

E296

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins

  • Western blot - Hsp90 beta antibody [E296] (ab32568)Western blot - Hsp90 beta antibody [E296] (ab32568) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Hsp90 beta antibody [E296] (ab32568)Immunohistochemistry (Paraffin-embedded sections) - Hsp90 beta antibody [E296] (ab32568) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500. Detects a ban...Read more →

    WB: 1/500. Detects a band of approximately 92 kDa (predicted molecular weight: 83 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50.

    IHC-P: 1/50.

  • ICC/IF

     ICC/IF: 1/50.

    ICC/IF: 1/50.

  • Flow Cyt

     Flow Cyt: 1/10.

    Flow Cyt: 1/10.

  • IP

     IP: 1/40.

    IP: 1/40.

Target

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Sequence similarities

Belongs to the heat shock protein 90 family.

Domain

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity.

Cellular localization

Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP08238 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 90 kda heat shock protein beta HSP90 beta antibody
  • D6S182 antibody
  • FLJ26984 antibody
  • Heat shock 84 kDa antibody
  • Heat shock 90kD protein 1, beta antibody
  • Heat shock 90kDa protein 1 beta antibody
  • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
  • Heat shock protein beta antibody
  • Heat shock protein HSP 90 beta antibody
  • Heat shock protein HSP 90-beta antibody
  • HS90B_HUMAN antibody
  • HSP 84 antibody
  • HSP 90 antibody
  • HSP84 antibody
  • HSP90 BETA antibody
  • hsp90ab1 antibody
  • HSP90B antibody
  • HSPC2 antibody
  • HSPCB antibody
see all

Database links

see all

Anti-Hsp90 beta antibody [E296] images:

  Western blot - Hsp90 beta antibody [E296] (ab32568)

Western blot - Hsp90 beta antibody [E296] (ab32568)

Anti-Hsp90 beta antibody [E296] (ab32568) at 1/500 dilution + Hela cell lysate

Predicted band size : 83 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - Hsp90 beta antibody [E296] (ab32568)

Immunohistochemistry (Paraffin-embedded sections) - Hsp90 beta antibody [E296] (ab32568)

Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using ab32568 at 1/50 dilution.

References for Anti-Hsp90 beta antibody [E296] (ab32568)

ab32568 has not yet been referenced specifically in any publications.

Publishing research using ab32568? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"