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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-H2AFZ (acetyl K3 + K8 + K10 + K14) antibody
See all H2AFZ products (4) ...
Description
Sheep polyclonal to H2AFZ (acetyl K3 + K8 + K10 + K14)
Specificity
ab43983 was raised against an acetylated N-terminal Htz1 peptide at residues K3, K8, K10 and K14. However, it is currently unclear if the antibody recognises all of these modifications or indeed unmodified Htz1. Customer feedback on this matter is appreciated.
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Does not react with
Chicken, Cow
Immunogen
Synthetic peptide: SGK(Ac)AHG GK(Ac)GK(A c)SGAK(Ac) DSGS-C, corresponding to N terminal amino acids 1-18 of Saccharomyces cerevisiae Htz1
SGK(Ac)AHG GK(Ac)GK(A c)SGAK(Ac) DSGS-C
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - Htz1 (acetyl K3 + K8 + K10 + K14) antibody (ab43983)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
Target
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similarities
Belongs to the histone H2A family.
Post-translational
modifications
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Not phosphorylated.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP0C0S5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H2A antibody
- H2A histone family member Z antibody
- h2a variant antibody
see all
Database links
- Entrez Gene: 854150 Saccharomyces cerevisiae
- SwissProt: Q12692 Saccharomyces cerevisiae
Anti-H2AFZ (acetyl K3 + K8 + K10 + K14) antibody images:
Western blot - Htz1 (acetyl K3 + K8 + K10 + K14) antibody (ab43983)
Anti-H2AFZ (acetyl K3 + K8 + K10 + K14) antibody (ab43983) at 2 µg/ml + S. cerevisiae lysate at 2 µg/ml
Predicted band size : 14 kDa
References for Anti-H2AFZ (acetyl K3 + K8 + K10 + K14) antibody (ab43983)
ab43983 has not yet been referenced specifically in any publications.
Publishing research using ab43983? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"