Htz1 antibody - ChIP Grade (ab4626)

Product Name 

Htz1 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Htz1 - ChIP Grade

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Saccharomyces cerevisiae Htz1.

(Peptide available as ab17826.)

Reacts with 
(species key)

Sc

Tested applications 
(see key)

ChIP, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IP: Use at an assay dependent dilution (from:17373856).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> Variants

Relevance 

Htz1 is the yeast version of H2A.Z, which functions in euchromatin to antagonize the spread of heterochromatin. The mechanism by which histone H2A is replaced by H2A.Z in the nucleosome is unknown. However, the recently isolated SWR1 complex catalyzes an ATP-dependent histone exchange with specificity for the histone variant H2A.Z. This provides a new theme in chromatin remodeling mechanisms and an explanation of how histone variants are incorporated into the nucleosome outside of S phase. In connection with the isolation of histone variant specific chaperone complexes, we are beginning to see the mechanisms that mix the histone octamer with intriguing implications for epigenetic inheritance.

During early mammalian development H2A.Z plays an essential, but unknown, function(s). It has been shown (Rangasamy et al., 2003) that undifferentiated mouse cells of the inner cell mass lack H2A.Z, but upon differentiation H2A.Z expression is switched on. Strikingly, H2A.Z is first targeted to pericentric hetero chromatin and then to other regions of the nucleus, but is excluded from the inactive X chromosome and the nucleolus.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Htz1 antibody - ChIP Grade images, references, products related to ab4626 and other tools.

Htz1 antibody - ChIP Grade images:

References for Htz1 antibody - ChIP Grade (ab4626)

  This product has been used in: (two most recent references)

Auger A et al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol 28:2257-70 (2008). ChIP; Saccharomyces cerevisiae. PubMed: 18212047

Altaf M et al. Interplay of chromatin modifiers on a short basic patch of histone H4 tail defines the boundary of telomeric heterochromatin. Mol Cell 28:1002-14 (2007). ChIP; Saccharomyces cerevisiae. PubMed: 18158898

See all 5 publication references for this product.


If you publish research using ab4626 please let us know so that we can cite the reference on this datasheet.

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