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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-H2AFZ antibody - ChIP Grade
See all H2AFZ products (4) ...
Description
Rabbit polyclonal to H2AFZ - ChIP Grade
Tested applications
ChIP, WB, IPmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Saccharomyces cerevisiae Htz1.
(Peptide available as ab17826.)
Positive control
This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> Variants
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent concentration.
WB: 1/500Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).(Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.)
IP: Use at an assay dependent dilution. (PubMed: 17373856)
Target
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similarities
Belongs to the histone H2A family.
Post-translational
modifications
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Not phosphorylated.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionP0C0S5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H2A antibody
- H2A histone family member Z antibody
- h2a variant antibody
see all
Database links
- Entrez Gene: 854150 Saccharomyces cerevisiae
- SwissProt: Q12692 Saccharomyces cerevisiae
Anti-H2AFZ antibody - ChIP Grade images:
Western blot - Anti-H2AFZ antibody (ab4626)
Anti-H2AFZ antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 20 minutes
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ChIP - Anti-Htz1 antibody (ab4626)
ab4626 used in ChIP.Nuclear lysate prepared from S cerevisiae S288c was used.Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.The detection step was performed using Real-time PCR.ab4626 was used at a 1/100 dilution for 4 hours at 4°C.
Image courtesy of Harsh Ja by Abreview.
References for Anti-H2AFZ antibody - ChIP Grade (ab4626)
This product has been referenced in:
- Papamichos-Chronakis Met al. Global regulation of H2A.Z localization by the INO80 chromatin-remodeling enzyme is essential for genome integrity. Cell 144:200-13 (2011). ChIP.Read more (PubMed: 21241891) »
- Gardner JMet al. Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z. J Cell Biol 193:489-507 (2011).Read more (PubMed: 21518795) »
See all 9 publications for this product
Publishing research using ab4626? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"