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Anti-H2AFZ antibody - ChIP Grade (ab4626)

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Overview

Product name

Anti-H2AFZ antibody - ChIP Grade
See all H2AFZ products (4) ...

Description

Rabbit polyclonal to H2AFZ - ChIP Grade

Tested applications

ChIP, WB, IPmore details

Cross reactivity

Reacts with

Saccharomyces cerevisiae

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Saccharomyces cerevisiae Htz1.

(Peptide available as ab17826.)

Positive control

This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Anti-H2AFZ antibody (ab4626)Western blot - Anti-H2AFZ antibody (ab4626) image (enlarge)

  • ChIP - Anti-Htz1 antibody (ab4626)ChIP - Anti-Htz1 antibody (ab4626) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Sequence similarities

Belongs to the histone H2A family.

Post-translational
modifications

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Not phosphorylated.

Cellular localization

Nucleus. Chromosome.

Target information above from: UniProt accessionP0C0S5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • H2A antibody
  • H2A histone family member Z antibody
  • h2a variant antibody
  • h2a.z antibody
  • H2A/z antibody
  • H2AFZ antibody
  • H2AV antibody
  • H2AZ antibody
  • H2AZ histone antibody
  • H2AZ_HUMAN antibody
  • Histone H2A.Z antibody
  • HTA3 antibody
  • Htz 1 antibody
  • MGC117173 antibody
  • OTTHUMP00000161678 antibody
see all

Database links

Anti-H2AFZ antibody - ChIP Grade images:

  Western blot - Anti-H2AFZ antibody (ab4626)

Western blot - Anti-H2AFZ antibody (ab4626)

Anti-H2AFZ antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 14 kDa
Observed band size : 14 kDa


Exposure time : 20 minutes

Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  ChIP - Anti-Htz1 antibody (ab4626)

ChIP - Anti-Htz1 antibody (ab4626)

ab4626 used in ChIP.

Nuclear lysate prepared from S cerevisiae S288c was used.
Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.
The detection step was performed using Real-time PCR.
ab4626 was used at a 1/100 dilution for 4 hours at 4°C.

Image courtesy of Harsh Ja by Abreview.

See Abreview

References for Anti-H2AFZ antibody - ChIP Grade (ab4626)

This product has been referenced in:

  • Papamichos-Chronakis Met al. Global regulation of H2A.Z localization by the INO80 chromatin-remodeling enzyme is essential for genome integrity. Cell 144:200-13 (2011). ChIP.Read more (PubMed: 21241891) »
  • Gardner JMet al. Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z. J Cell Biol 193:489-507 (2011).Read more (PubMed: 21518795) »

See all 9 publications for this product

Publishing research using ab4626? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"