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Product Name
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Htz1 antibody - ChIP Grade
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See all Htz1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Htz1 - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Saccharomyces cerevisiae Htz1.
(Peptide available as ab17826.)
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Reacts with
(species key)
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Sc
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Tested applications
(see key)
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ChIP: Use at an assay dependent dilution. WB: 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). IP: Use at an assay dependent dilution (from:17373856).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies Chromatin and Nuclear Signaling >> Histones >> Variants
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Relevance
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Htz1 is the yeast version of H2A.Z, which functions in euchromatin to antagonize the spread of heterochromatin. The mechanism by which histone H2A is replaced by H2A.Z in the nucleosome is unknown. However, the recently isolated SWR1 complex catalyzes an ATP-dependent histone exchange with specificity for the histone variant H2A.Z. This provides a new theme in chromatin remodeling mechanisms and an explanation of how histone variants are incorporated into the nucleosome outside of S phase. In connection with the isolation of histone variant specific chaperone complexes, we are beginning to see the mechanisms that mix the histone octamer with intriguing implications for epigenetic inheritance.
During early mammalian development H2A.Z plays an essential, but unknown, function(s). It has been shown (Rangasamy et al., 2003) that undifferentiated mouse cells of the inner cell mass lack H2A.Z, but upon differentiation H2A.Z expression is switched on. Strikingly, H2A.Z is first targeted to pericentric hetero chromatin and then to other regions of the nucleus, but is excluded from the inactive X chromosome and the nucleolus.
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Htz1 antibody - ChIP Grade (ab4626)
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 854150 (Saccharomyces cerevisiae)
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| SwissProt | | Q12692 (Saccharomyces cerevisiae)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Htz1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Htz1 antibody - ChIP Grade images, references, products related to ab4626 and other tools.
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Htz1 antibody - ChIP Grade images:
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All lanes : Htz1 antibody - ChIP Grade (ab4626) at 1/500 dilution
Lane 1 : S. cerevisiae lysate. Lane 2 : S. cerevisiae lysate. with Htz1 peptide (ab17826) at 1 mg/ml
Secondary Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 14 kDa Observed band size : 18 kDa (why is the actual band size different from the predicted?) Additional bands at : 105 kDa (possible cross reactivity),55 kDa (possible cross reactivity),60 kDa (possible cross reactivity).
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References for Htz1 antibody - ChIP Grade (ab4626)
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This product has been used in: (two most recent references)
Auger A et al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol 28:2257-70 (2008). ChIP; Saccharomyces cerevisiae. PubMed: 18212047
Altaf M et al. Interplay of chromatin modifiers on a short basic patch of histone H4 tail defines the boundary of telomeric heterochromatin. Mol Cell 28:1002-14 (2007). ChIP; Saccharomyces cerevisiae. PubMed: 18158898
See all 5 publication references for this product.
If you publish research using ab4626 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Htz1
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