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Product Name
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Htz1 peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide derived from within residues 1 - 100 of S. cerevisiae Htz1. .
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> Variants
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Relevance
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Htz1 is the yeast version of H2A.Z, which functions in euchromatin to antagonize the spread of heterochromatin. The mechanism by which histone H2A is replaced by H2A.Z in the nucleosome is unknown. However, the recently isolated SWR1 complex catalyzes an ATP-dependent histone exchange with specificity for the histone variant H2A.Z. This provides a new theme in chromatin remodeling mechanisms and an explanation of how histone variants are incorporated into the nucleosome outside of S phase. In connection with the isolation of histone variant specific chaperone complexes, we are beginning to see the mechanisms that mix the histone octamer with intriguing implications for epigenetic inheritance.
During early mammalian development H2A.Z plays an essential, but unknown, function(s). It has been shown (Rangasamy et al., 2003) that undifferentiated mouse cells of the inner cell mass lack H2A.Z, but upon differentiation H2A.Z expression is switched on. Strikingly, H2A.Z is first targeted to pericentric hetero chromatin and then to other regions of the nucleus, but is excluded from the inactive X chromosome and the nucleolus.
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, HEPES, 2mM EDTA, 150mM Sodium chloride
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Expression system
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CHO
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Htz1 peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Htz1 peptide images, references, products related to ab17826 and other tools.
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Htz1 peptide images:
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All lanes : Htz1 antibody - ChIP Grade (ab4626) at 1/500 dilution
Lane 1 : S. cerevisiae lysate.
Lane 2 : S. cerevisiae lysate. with Htz1 peptide (ab17826) at 1 mg/ml
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa (possible cross reactivity),55 kDa (possible cross reactivity),60 kDa (possible cross reactivity).
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Search PubMed (MEDLINE) for references to Htz1
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Htz1 peptide - more information
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Customer reviews (feedback) regarding Htz1 peptide |
Customer FAQs regarding Htz1 peptide |
Protocols for Htz1 peptide |
Price and availability of products related to Htz1 peptide |
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Htz1 peptide - related products:
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Htz1 peptide - other tools:
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Contact Abcam with a Technical Enquiry about Htz1 peptide |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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