Anti-HuD antibody (ab96474)
Key features and details
- Rabbit polyclonal to HuD
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-HuD antibody
See all HuD primary antibodies -
Description
Rabbit polyclonal to HuD -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Macaque monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Mouse HuD aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab151535) -
Positive control
- This antibody gave a positive signal within Western Blot in the following tissue lysates: Mouse Brain P7; Rat Brain P7; Mouse Brain P0; Rat Brain P0. This antibody gave a positive result in IHC in the following FFPE tissue: Human hippocampus.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
IHC-P |
Use a concentration of 5 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam recommends using milk as the blocking agent (3%).
|
Notes |
---|
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam recommends using milk as the blocking agent (3%). |
Target
-
Function
May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth. - Information by UniProt
-
Database links
- Entrez Gene: 1996 Human
- Entrez Gene: 15572 Mouse
- Entrez Gene: 432358 Rat
- Omim: 168360 Human
- SwissProt: P26378 Human
- SwissProt: Q61701 Mouse
- SwissProt: O09032 Rat
- Unigene: 213050 Human
see all -
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
- ELAV L4 antibody
- ELAV like 4 antibody
see all
Images
-
All lanes : Anti-HuD antibody (ab96474) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lane 2 : P7 Rat Brain Rat Tissue Lysate
Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
Lane 4 : P0 Rat Brain Rat Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 40,42 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab96474 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
ICC/IF image of ab96474 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96474 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
IHC image of HuD staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab96474 has been referenced in 3 publications.
- Olguin SL et al. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization. Commun Biol 5:672 (2022). PubMed: 35798971
- Mustaciosu CC et al. RNA-Binding Proteins HuB, HuC, and HuD are Distinctly Regulated in Dorsal Root Ganglia Neurons from STZ-Sensitive Compared to STZ-Resistant Diabetic Mice. Int J Mol Sci 20:N/A (2019). PubMed: 31013625
- McCleskey EW & Gold MS Ion channels of nociception. Annu Rev Physiol 61:835-56 (1999). PubMed: 10099712