Overview

  • Product name
    Human ADAMTS13 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 5.62ng/ml 0.259 4.6%
    2 16 10.1ng/ml 0.444 4.4%
    3 16 25.5ng/ml 1.07 4.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 6.23ng/ml 0.467 7.5%
    2 24 12.1ng/ml 0.835 6.9%
    3 24 26.4ng/ml 1.95 7.4%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human ADAMTS13 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213752) is designed for the quantitative measurement of Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been pre-coated onto 96-well plates. Standards (CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS13 amount of sample captured in plate.

  • Notes

    ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. It is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Human ADAMTS13 coated Microplate (12 x 8 wells) 1 unit
    Lyophilized recombinant Human ADAMTS13 standard 2 vials
    Biotinylated anti- Human ADAMTS13 antibody 1 x 130µl
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Sample Diluent Buffer 1 x 30ml
    Antibody Diluent Buffer 1 x 12ml
    ABC Diluent Buffer 1 x 12ml
    TMB 1 x 10ml
    Stop solution 1 x 10ml
    Adhesive Plate Seal 4 units
  • Research areas
  • Function
    Cleaves the vWF multimers in plasma into smaller forms.
  • Tissue specificity
    Plasma. Expressed primarily in liver.
  • Involvement in disease
    Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
  • Sequence similarities
    Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.
  • Domain
    The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
  • Post-translational
    modifications
    May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 13
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13
    • ADAM metallopeptidase with thrombospondin type 1 motif 13
    • ADAM TS
    • ADAM-TS 13
    • ADAM-TS13
    • ADAMTS 13
    • ADAMTS-13
    • ADAMTS13
    • ADAMTS13 protein
    • ATS13_HUMAN
    • C9orf8
    • TTP
    • Von Willebrand factor cleaving protease
    • von Willebrand factor-cleaving protease
    • vWF cleaving protease
    • vWF CP
    • vWF-cleaving protease
    • vWF-CP
    • vWFCP
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human ADAMTS13 ELISA Kit (ab213752) Standard Curve.

Protocols

References

ab213752 has not yet been referenced specifically in any publications.

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