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The Human ADAMTS13 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213752) is designed for the quantitative measurement of Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been pre-coated onto 96-well plates. Standards (CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS13 amount of sample captured in plate.
ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. It is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
|Components||1 x 96 tests|
|Anti-Human ADAMTS13 coated Microplate (12 x 8 wells)||1 unit|
|Lyophilized recombinant Human ADAMTS13 standard||2 vials|
|Biotinylated anti- Human ADAMTS13 antibody||1 x 130µl|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Sample Diluent Buffer||1 x 30ml|
|Antibody Diluent Buffer||1 x 12ml|
|ABC Diluent Buffer||1 x 12ml|
|TMB||1 x 10ml|
|Stop solution||1 x 10ml|
|Adhesive Plate Seal||4 units|
Our Abpromise guarantee covers the use of ab213752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab213752 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"