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Question (75732) | Human C Reactive Protein ELISA Kit (ab181416)

Thanks Healther. With your help I was able to solve most of the questions. However for IgG Human ELISA kit (ab100547), the dilution is 10,000,000 folds. The sample diluent solution is also used for std, HRP-streptaridin and antibody dilution. Is there anything that can replace the Assay diluent?

The lab has done some further testing with diluents and I wanted to update you.

We can confirm that the 1X Wash buffer can be used to pre-dilute the biological sample 500 X. Another possibility is to use a mixture of 1X Wash Buffer and Sample Diluent NS for the pre-dilutions. The final dilution (that is added to the plate) should be made always in Sample diluent NS.

Please let me know if you have any further questions!