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Thanks Healther. With your help I was able to solve most of the questions. However for IgG Human ELISA kit (ab100547), the dilution is 10,000,000 folds. The sample diluent solution is also used for std, HRP-streptaridin and antibody dilution. Is there anything that can replace the Assay diluent?
Asked on Apr 17 2014
The lab has done some further testing with diluents and I wanted to update you.
We can confirm that the 1X Wash buffer can be used to pre-dilute the biological sample 500 X. Another possibility is to use a mixture of 1X Wash Buffer and Sample Diluent NS for the pre-dilutions. The final dilution (that is added to the plate) should be made always in Sample diluent NS.
Please let me know if you have any further questions!
Heather Allen Abcam Scientific Support
Answered on Apr 17 2014