Human CD200 ELISA Kit (ab155429)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.025 ng/ml - 6 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD200 ELISA Kit
See all CD200 / OX2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
0.025 ng/ml - 6 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 86.14 77% - 95% Plasma = 78.35 67% - 86% Cell culture media = 81.85 74% - 89% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CD200 Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD200 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD200 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD200 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD200 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 240X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human CD200 2 vials CD200 Microplate (12 x 8 wells) 1 unit Recombinant Human CD200 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2
- CD200
- CD200 antigen
see all -
Database links
- Entrez Gene: 4345 Human
- Omim: 155970 Human
- SwissProt: P41217 Human
- Unigene: 79015 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155429 has not yet been referenced specifically in any publications.