Overview

  • Product name
    Human Complement Factor I ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    0.25 µg/ml
  • Range
    0.375 µg/ml - 24 µg/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement Factor I Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement Factor I levels in plasma, serum, milk, saliva, and cell culture supernatant.

    A Complement Factor I specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement Factor I is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement Factor I captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    Biotinylated Complement Factor I (Lyophilised) 1 vial
    Chromogen Substrate 1 x 8ml
    Complement Factor I Microplate (12 x 8 well strips) 1 unit
    Complement Factor I Standard (Lyophilised) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Function
    Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Hemolytic uremic syndrome atypical 3
    Complement factor I deficiency
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 Kazal-like domain.
    Contains 2 LDL-receptor class A domains.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • AHUS3
    • ARMD13
    • C3b INA
    • C3b inactivator
    • C3B/C4B inactivator
    • C3BINA
    • CFAI_HUMAN
    • Cfi
    • Complement component I
    • Complement control protein factor I
    • Complement factor I heavy chain
    • Complement factor I light chain
    • FI
    • IF
    • KAF
    • Konglutinogen activating factor
    • Light chain of factor I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab195460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab195460.

Protocols

References

This product has been referenced in:
  • Wu B  et al. Plasma Levels of Complement Factor I and C4b Peptides Are Associated with HIV Suppression. ACS Infect Dis 3:880-885 (2017). Read more (PubMed: 28862830) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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