Human Cystatin C ELISA Kit (ab119589)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 0.312 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cystatin C ELISA Kit
See all Cystatin C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 0.687ng/ml 0.044 = 6.4% 2 16 2.837ng/ml 0.207 = 7.3% 3 16 10.374ng/ml 0.684 = 6.6% Inter-assay Sample n Mean SD CV% 1 24 0.647ng/ml 0.051 = 7.9% 2 24 2.715ng/ml 0.217 = 8% 3 24 10.112ng/ml 0.859 = 8.5% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
0.312 ng/ml - 20 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.
A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.
Get results in 90 minutes with Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Cystatin C antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human Cystatin C antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human Cystatin C standard 2 x 20ng 2 x 20ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
Involvement in disease
Amyloidosis 6
Macular degeneration, age-related, 11 -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
Database links
- Entrez Gene: 1471 Human
- Omim: 604312 Human
- SwissProt: P01034 Human
- Unigene: 304682 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119589 has been referenced in 1 publication.
- Natarajan S et al. Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker. Sensors (Basel) 21:N/A (2021). PubMed: 34063596