Overview

  • Product name
    Human FABP2 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Serum, Plasma, Cell culture media
  • Assay type
    Sandwich
  • Sensitivity
    25 pg/ml
  • Range
    25 pg/ml - 100000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 129.8 120% - 136%
    Plasma 126.5 110% - 135%
    Cell culture media 112.4 103% - 123%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.

    This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    1500X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    Assay Diluent B. 5x concentrated buffer. For detection antibody and HRP-Streptavidin diluent. 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated Human FABP2 detection antibody (lyophilized) 2 vials
    Human FABP2 Standards 2 vials
    Pre-coated Human FABP2 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificity
    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • FABP2
    • FABPI_HUMAN
    • Fatty acid-binding protein
    • Fatty acid-binding protein 2
    • I-FABP
    • intestinal
    • Intestinal-type fatty acid-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human FABP2 ELISA Kit images

Protocols

References for Human FABP2 ELISA Kit (ab193700)

ab193700 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
After trying numerous porcine-specific ELISAs for FABP2, I decided to risk trying this ELISA in hopes that it would give satisfactory results. As the title says, it works well with a good standard curve and a very low background. My samples were diluted 1:1 with the sample dilution buffer provided, which yielded good results.

Duplication of samples were very close, and there were no problems with the plate.

As an aside, the standard curve in the mixture was not plated in order, so it appears incorrect, however it worked quite well.
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Submitted Mar 29 2017

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