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|Sample type||Average %||Range|
|Serum||129.8||120% - 136%|
|Plasma||126.5||110% - 135%|
|Cell culture media||112.4||103% - 123%|
Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|1500X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|Assay Diluent B. 5x concentrated buffer. For detection antibody and HRP-Streptavidin diluent.||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated Human FABP2 detection antibody (lyophilized)||2 vials|
|Human FABP2 Standards||2 vials|
|Pre-coated Human FABP2 Microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193700 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"