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|Sample type||Average %||Range|
|Serum||96||92% - 101%|
|Heparin Plasma||99||99% - 100%|
|EDTA Plasma||93||91% - 98%|
|Citrate Plasma||93||91% - 97%|
|Extraction Buffer||96||91% - 100%|
Glutathione Peroxidase 1 (GPX1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Glutathione Peroxidase 1 protein in human serum, plasma, cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
GPX1 is a selenium-containing enzyme part of the glutathione system responsible for protecting against oxidative damage by catalyzing the reduction of hydroperoxides with glutathione. The homotetramer, GPX1, utilizes its selenocysteine-containing active site to primarily scavenge hydrogen peroxide. The dimerization of glutathione ultimately provides the electrons to reduce the active site, restoring GPX1’s activity. As a critical component of the regulation of oxidative species within cells, GPX1 is directly involved in metabolism, various cancers, and oxidative stress. This assay is specific to isoform 1 of glutathione peroxidase.
|Components||1 x 96 tests|
|10X GPX1 Capture Antibody||1 x 600µl|
|10X GPX1 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|Antibody Diluent CPI - HAMA Blocker (ab193969)||1 x 6ml|
|GPX1 Human Lyophilized Recombinant Protein||2 vials|
|Plate Seal||1 unit|
|Sample Diluent 75BP||1 x 20ml|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab193767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
ab193767 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"