Anti-Human Growth Hormone antibody [GH-1] (ab9821)
- Product nameAnti-Human Growth Hormone antibody [GH-1]See all Human Growth Hormone primary antibodies ...
- DescriptionMouse monoclonal [GH-1] to Human Growth Hormone
- Tested applicationsELISA, Indirect ELISA, WB, Sandwich ELISA more details
- Species reactivityReacts with: Human
Recombinant full length protein (Human).
- EpitopeAb9821 recognizes a different epitope than GH-2 (ab9822).
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.1% sodium azide, pH 7.4
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone numberGH-1
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab9821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||I-ELISA: 1/1000 - 1/10000.|
|WB||WB: 1/500 - 1/2000. Predicted molecular weight: 22-24 kDa.|
|Sandwich ELISA||sELISA: Use at an assay dependent dilution.|
- FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
- Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
- Sequence similaritiesBelongs to the somatotropin/prolactin family.
- Cellular localizationSecreted.
- GH 1 antibodyGH antibodyGH N antibody
- GH-N antibodyGH1 antibodyGHN antibodyGHN antibodyGrowth Hormone 1 antibodyGrowth hormone 1 variant 1 antibodyGrowth hormone 1 variant 2 antibodyGrowth hormone antibodyGrowth Hormone Normal antibodyGrowth Hormone Pituitary antibodyGrowth hormone, normal antibodyGrowth hormone, pituitary antibodyHG1 antibodyHG1 antibodyhGH N antibodyhGH-N antibodyHGHN antibodyIGHD1B antibodyPituitary Growth Hormone antibodyRNGHGP antibodySOMA_HUMAN antibodySomatotropin C antibodySomatotropin antibodySomatotropin I Precursor antibody
Anti-Human Growth Hormone antibody [GH-1] images
All lanes : Anti-Human Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution
Lane 1 :
Human Growth Hormone protein (ab51232) at 0.1 µg
Lane 2 :
Human Growth Hormone protein (ab51232) at 0.01 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
Predicted band size : 22-24 kDa
Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.
References for Anti-Human Growth Hormone antibody [GH-1] (ab9821)
This product has been referenced in:
- Rezaei B et al. Immobilization of specific monoclonal antibody on Au nanoparticles for hGH detection by electrochemical impedance spectroscopy. Biosens Bioelectron 25:395-9 (2009). Read more (PubMed: 19692224) »