Anti-Growth Hormone antibody [GH-1] (ab9821)
Key features and details
- Mouse monoclonal [GH-1] to Growth Hormone
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Growth Hormone antibody [GH-1]
See all Growth Hormone primary antibodies -
Description
Mouse monoclonal [GH-1] to Growth Hormone -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Growth Hormone.
Database link: P01241 -
Epitope
Ab9821 recognizes a different epitope than GH-2 (ab9822). -
General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
GH-1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 22-24 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 22-24 kDa. |
Target
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Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. -
Involvement in disease
Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
Sequence similarities
Belongs to the somatotropin/prolactin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2688 Human
- Omim: 139250 Human
- SwissProt: P01241 Human
- Unigene: 655229 Human
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Alternative names
- gH antibody
- GH-N antibody
- GH1 antibody
see all
Images
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All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution
Lane 1 :Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg
Lane 2 :Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22-24 kDa
Exposure time: 2 minutes -
Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab9821 has been referenced in 6 publications.
- Sleumer B et al. Selective quantification of the 22-kDa isoform of human growth hormone 1 in serum and plasma by immunocapture and LC-MS/MS. Anal Bioanal Chem 414:6187-6200 (2022). PubMed: 35838770
- Bults P et al. Intact protein quantification in biological samples by liquid chromatography - high-resolution mass spectrometry: somatropin in rat plasma. J Chromatogr B Analyt Technol Biomed Life Sci 1144:122079 (2020). PubMed: 32247186
- Liu Y et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int J Mol Med 42:3037-3046 (2018). PubMed: 30272367
- Sun H et al. A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis. Nat Commun 7:10339 (2016). PubMed: 26842342
- Kunert R et al. CHO-recombinant human growth hormone as a protease sensitive reporter protein. Appl Microbiol Biotechnol 84:693-9 (2009). PubMed: 19396439
- Rezaei B et al. Immobilization of specific monoclonal antibody on Au nanoparticles for hGH detection by electrochemical impedance spectroscopy. Biosens Bioelectron 25:395-9 (2009). PubMed: 19692224