Anti-Human Growth Hormone antibody [SPM106] (ab15317)

Overview

• Product nameAnti-Human Growth Hormone antibody [SPM106]See all Human Growth Hormone primary antibodies ...
• Description
  Mouse monoclonal [SPM106] to Human Growth Hormone
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified human growth hormone

• Positive controlAnterior pituitary

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.05% Sodium azide
  Constituents: BSA, 0.0268% PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberSPM106
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Post embryonic development
  • Growth hormones

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

Applications

Target

• FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
• Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
  Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
  Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
  Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
• Sequence similaritiesBelongs to the somatotropin/prolactin family.
• Cellular localizationSecreted.

Target information above from: UniProt accession P01241 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2688 Human
  • Omim: 139250 Human
  • SwissProt: P01241 Human
  • Unigene: 655229 Human

• Alternative names
  GH 1 antibodyGH antibodyGH N antibody

  GH-N antibodyGH1 antibodyGHN antibodyGHN antibodyGrowth Hormone 1 antibodyGrowth hormone 1 variant 1 antibodyGrowth hormone 1 variant 2 antibodyGrowth hormone antibodyGrowth Hormone Normal antibodyGrowth Hormone Pituitary antibodyGrowth hormone, normal antibodyGrowth hormone, pituitary antibodyHG1 antibodyHG1 antibodyhGH N antibodyhGH-N antibodyHGHN antibodyIGHD1B antibodyPituitary Growth Hormone antibodyRNGHGP antibodySOMA_HUMAN antibodySomatotropin C antibodySomatotropin antibodySomatotropin I Precursor antibody

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Anti-Human Growth Hormone antibody [SPM106] images

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  - Human Growth Hormone antibody [SPM106] (ab15317)

  Human anterior pituitary stained with ab15317

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  Immunocytochemistry/ Immunofluorescence - Anti-Human Growth Hormone antibody [SPM106] (ab15317)Image from Vakili H et al., Molecular and Cellular Endocrinology: Volume 345, Issues 1–2, 15 October 2011, Pages 48–57. Fig 2.; doi: 10.1016/j.mce.2011.07.010. Epub 2011 Jul 12. with permission from Elsevier.
  Immunofluorescence analysis of Human Growth Hormone producing pituitary cells from transgenic mice, staining Human Growth Hormone with ab15317.
  
  Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/200) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with Cy3-conjugated anti-mouse secondary antibody (1/330) for 1 hour at room temperature.