Anti-Human Growth Hormone antibody [SPM106] (ab15317)
- Product nameAnti-Human Growth Hormone antibody [SPM106]See all Human Growth Hormone primary antibodies ...
- DescriptionMouse monoclonal [SPM106] to Human Growth Hormone
- Tested applicationsICC/IF, WB, IHC-P more details
- Species reactivityReacts with: Human
Purified human growth hormone
- Positive controlAnterior pituitary
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: BSA, 0.0268% PBS
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberSPM106
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab15317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration. PubMed: 21777655|
|WB||WB: Use at an assay dependent concentration. PubMed: 21777655|
|IHC-P||IHC-P: 1/200. 1/200 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
- FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
- Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
- Sequence similaritiesBelongs to the somatotropin/prolactin family.
- Cellular localizationSecreted.
- GH 1 antibodyGH antibodyGH N antibody
- GH-N antibodyGH1 antibodyGHN antibodyGHN antibodyGrowth Hormone 1 antibodyGrowth hormone 1 variant 1 antibodyGrowth hormone 1 variant 2 antibodyGrowth hormone antibodyGrowth Hormone Normal antibodyGrowth Hormone Pituitary antibodyGrowth hormone, normal antibodyGrowth hormone, pituitary antibodyHG1 antibodyHG1 antibodyhGH N antibodyhGH-N antibodyHGHN antibodyIGHD1B antibodyPituitary Growth Hormone antibodyRNGHGP antibodySOMA_HUMAN antibodySomatotropin C antibodySomatotropin antibodySomatotropin I Precursor antibody
Anti-Human Growth Hormone antibody [SPM106] images
Human anterior pituitary stained with ab15317
Immunofluorescence analysis of Human Growth Hormone producing pituitary cells from transgenic mice, staining Human Growth Hormone with ab15317.
Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/200) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with Cy3-conjugated anti-mouse secondary antibody (1/330) for 1 hour at room temperature.
References for Anti-Human Growth Hormone antibody [SPM106] (ab15317)
This product has been referenced in:
- Vakili H et al. Transgenic mice expressing the human growth hormone gene provide a model system to study human growth hormone synthesis and secretion in non-tumor-derived pituitary cells: differential effects of dexamethasone and thyroid hormone. Mol Cell Endocrinol 345:48-57 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21777655) »