Human IL-10 ELISA Kit (ab100549)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 2.34 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
2.34 pg/ml - 150 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.37 87% - 110% Serum 101.94 87% - 108% Plasma 102.39 93% - 109% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.
Produced using a non-baculovirus expression system.
-
Notes
Optimization may be required with urine samples
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-10 2 vials IL-10 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
Associated products
-
SimpleStep ELISA kits
Images
-
IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).
-
Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).
-
Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).
-
Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).
-
Representative standard curve using ab100549
-
Representative standard curve using ab100549
Datasheets and documents
-
SDS download
-
Datasheet download
References (18)
ab100549 has been referenced in 18 publications.
- Xu W et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
- Zhou T et al. microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis. Aging (Albany NY) 13:11061-11082 (2021). PubMed: 33744854
- Flaherty RA et al. Distinct Group B Streptococcus Sequence and Capsule Types Differentially Impact Macrophage Stress and Inflammatory Signaling Responses. Infect Immun 89:N/A (2021). PubMed: 33558317
- Sun W et al. The Prognostic Determinant of Interleukin-10 in Patients with Acute Ischemic Stroke: An Analysis from the Perspective of Disease Management. Dis Markers 2021:6423244 (2021). PubMed: 34336007
- El-Ali Z et al. Evaluation of circulating levels of Interleukin-10 and Interleukin-16 and dietary inflammatory index in Lebanese knee osteoarthritis patients. Heliyon 7:e07551 (2021). PubMed: 34337179