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|Sample type||Average %||Range|
|Serum||82||77% - 84%|
|Cell culture media||100||97% - 103%|
|EDTA Plasma||82||80% - 85%|
|Citrate Plasma||84||81% - 85%|
Human IL6 SimpleStep ELISA® kit (ab178013) has been re-developed with new capture and detector antibodies. We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying IL6 in human serum, plasma and cell culture supernatants. All orders placed after 11th December 2017/Lot number GR3193751-2 will be fulfilled with kits containing the new recombinant antibodies.
IL6 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL6 protein in human serum, plasma and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
|Components||1 x 96 tests|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 5BI||1 x 6ml|
|Human IL6 Capture Antibody Lyophilized||1 vial|
|Human IL6 Detector Antibody 10X||1 x 600µl|
|Human IL6 Lyophilized Recombinant||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab178013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve comparison between the previous Human IL6 SimpleStep ELISA® kit and the re-developed Human IL6 SimpleStep ELISA® kit. The re-developed kit shows increased sensitivity.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell culture supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"