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Product Name
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Human Ig light chain antibody [MHK-49] (Biotin)
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See all Human Ig light chain antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MHK-49] to Human Ig light chain (Biotin)
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Immunogen
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Full length proteins -a Human IgGs, IgM, IgE, and IgA cocktail
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.
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Tested applications
(see key)
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Flow Cyt
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use <0.5µg for 106 cells in 100µl staining volume or 100µl of whole blood.
This antibody can be used as primary or secondary reagent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane: single-pass type I membrane protein and Secreted
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Research areas
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Immunology >> Immunoglobulins >> Light Chain >> Lambda
Immunology >> Immunoglobulins >> Light Chain >> Kappa
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Relevance
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There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain.
In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species.
Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MHK-49
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Conjugation notes
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The solution is free of unconjugated biotin.
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Biotin
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Human Ig light chain antibody [MHK-49] (Biotin) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Human Ig light chain
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