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Products:Immunology >> Immunoglobulins >> Light Chain >> Kappa
Overview
Product name
Anti-Human Ig light chain antibody [MHK-49]
See all Human Ig light chain products (5) ...
Description
Mouse monoclonal [MHK-49] to Human Ig light chain
Specificity
This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.
Tested applications
ICC/IF, ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Human IgGs, IgM, IgE, IgA cocktail
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
MHK-49
Isotype
IgG1
Light chain type
kappa
Research areas
Immunology >> Immunoglobulins >> Light Chain >> Lambda
Immunology >> Immunoglobulins >> Light Chain >> Kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. (This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.)
Flow Cyt: Use 0.5µg for 106 cells.
Target
Relevance
There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
Cellular localization
Cell Membrane: single-pass type I membrane protein and Secreted
Alternative names
- MGC111575 antibody
- HCAK1 antibody
- Ig kappa chain antibody
see all
Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
- SwissProt: Q502W4 Human
- SwissProt: Q569I9 Human
- SwissProt: Q6GMV9 Human
- SwissProt: Q6GMW1 Human
- SwissProt: Q6GMX8 Human
see all
References for Anti-Human Ig light chain antibody [MHK-49] (ab48536)
ab48536 has not yet been referenced specifically in any publications.
Publishing research using ab48536? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"