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Product Name
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Human Ig light chain antibody [MHK-49]
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See all Human Ig light chain antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MHK-49] to Human Ig light chain
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Immunogen
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Human IgGs, IgM, IgE, IgA cocktail
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC/IF
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use <0.5µg for 106 cells in 100µl staining volume or 100µl of whole blood.
ICC/IF: Use at an assay dependent dilution.
This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane: single-pass type I membrane protein and Secreted
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Research areas
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Immunology >> Immunoglobulins >> Light Chain >> Lambda
Immunology >> Immunoglobulins >> Light Chain >> Kappa
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Relevance
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There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain.
In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species.
Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MHK-49
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Human Ig light chain antibody [MHK-49] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Human Ig light chain
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Human Ig light chain antibody [MHK-49] - more information
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