Overview

  • Product nameAnti-Human IgE antibody [4G7]See all Human IgE primary antibodies ...
  • Description
    Mouse monoclonal [4G7] to Human IgE
  • SpecificityThis antibody reacts with human IgE. Specificity was confirmed by ELISA
  • Tested applicationsELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • General notesThis antibody recognizes an epitope different from the one recognized by ab774 and ab775.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by DEAE chromatography and precipitation methods.
  • Clonality Monoclonal
  • Clone number4G7
  • Myelomaunknown
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
  • Application notesELISA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Ig epsilon chain C region antibody
      • IGHE antibody
      • Immunoglobulin epsilon antibody
      • Immunoglobulin heavy constant epsilon antibody

    References for Anti-Human IgE antibody [4G7] (ab773)

    This product has been referenced in:
    • Mueller GA  et al. Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem 276:9359-65 (2001). Read more (PubMed: 11134039) »
    • Smith AM  et al. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol 107:977-84 (2001). Read more (PubMed: 11398074) »

    See all 2 Publications for this product

    Product Wall

    Detailed epitope mapping was not performed (only cross-blocking studies with the three mAb to human IgE). Conjugates with HRP will be available, most probably will appear in the catalogue on September/October 2002. The preliminary studies showed that H...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"