Anti-Human IgG antibody [E20V] (ab180555)
Key features and details
- Rabbit monoclonal [E20V] to Human IgG
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Human IgG antibody [E20V]
See all Human IgG primary antibodies -
Description
Rabbit monoclonal [E20V] to Human IgG -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Human IgG (internal sequence). The exact sequence is proprietary.
Database link: P01857 -
Epitope
Antibody recognizes the epitope located between Val167 - Val185 -
Positive control
- Human plasma cells in tonsillar tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 2% BSA, 0.32% Tris HCl -
Concentration information loading...
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Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. ab180555 is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
E20V -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab180555 has not yet been referenced specifically in any publications.