Recombinant Anti-Human IgG antibody [IG266] (ab200699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IG266] to Human IgG
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Human IgG antibody [IG266]
See all Human IgG primary antibodies -
Description
Mouse monoclonal [IG266] to Human IgG -
Host species
Mouse -
Specificity
ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.
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Positive control
- IHC-P: Human colon and tonsil tissue. Flow cyto: Human peripheral blood mononuclear cell (PBMC) cells WB: Human serum, human spleen and tonsil tissue lysates
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General notes
This product has switched from a hybridoma to recombinant production method on 14th March 2024.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
IG266 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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Flow Cyt |
1/800.
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IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Predicted molecular weight: 36 kDa. |
Flow Cyt
1/800. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining in plasmocyte of human tonsil.
The section was incubated with ab200699 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Cytoplasmic staining in plasma of human colon.
The section was incubated with ab200699 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Human IgG with ab200699 at 1/800 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.
Goat anti mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab200699. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells. -
All lanes : Anti-Human IgG antibody [IG266] (ab200699) at 1/1000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 6 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Anti-Human IgG antibody [IG266] (ab200699) at 1/5000 dilution + Human serum at 10 µg
Secondary
Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab200699 has been referenced in 34 publications.
- Zhao Y et al. Antidepressant-like effects of geniposide in chronic unpredictable mild stress-induced mice by regulating the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 networks. Phytother Res 37:1850-1863 (2023). PubMed: 36515407
- Chen Q et al. Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia. J Transl Med 21:496 (2023). PubMed: 37488572
- Wang M et al. Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes. Mol Med 29:130 (2023). PubMed: 37740187
- Hui Z et al. Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC). Cell Death Dis 13:607 (2022). PubMed: 35831283
- Sun X et al. Long non‑coding RNA CASC11 interacts with YBX1 to promote prostate cancer progression by suppressing the p53 pathway. Int J Oncol 61:N/A (2022). PubMed: 35904175