Anti-Human IgG antibody [KT131] (ab211339)
Key features and details
- Mouse monoclonal [KT131] to Human IgG
- Suitable for: Sandwich ELISA, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-Human IgG antibody [KT131]
See all Human IgG primary antibodies -
Description
Mouse monoclonal [KT131] to Human IgG -
Host species
Mouse -
Specificity
ab211339 is specific for IgG1, IgG2, IgG3, IgG4. It does not cross-react with Human IgA, IgE and IgM. -
Tested applications
Suitable for: Sandwich ELISA, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length protein corresponding to Human Human IgG.
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Positive control
- Purified immunoglobulins IgG1, IgG2, IgG3, IgG4, IgG.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KT131 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/10000.
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ELISA |
1/1000.
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Notes |
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Sandwich ELISA
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/10000. |
ELISA
1/1000. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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All lanes : Anti-Human IgG antibody [KT131] (ab211339) at 1/500 dilution
Lane 1 : Purified immunoglobulin IgG1
Lane 2 : Purified immunoglobulin IgG2
Lane 3 : Purified immunoglobulin IgG3
Lane 4 : Purified immunoglobulin IgG4
Lane 5 : Purified immunoglobulin IgG
Lane 6 : Purified immunoglobulin IgA
Lane 7 : Purified immunoglobulin IgE
Lane 8 : Purified immunoglobulin IgM -
Sandwich ELISA for normal Human serum samples using ab211339 coated plate and HRP conjugated antibody.
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Sandwich ELISA for purified IgG using ab211339 coated plate and HRP conjugated antibody.
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ELISA using plates coated with Human antibodies with various isotypes.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab211339 has been referenced in 1 publication.
- Wang L & Ji S Inhibition of Ubc9-Induced CRMP2 SUMOylation Disrupts Glioblastoma Cell Proliferation. J Mol Neurosci 69:391-398 (2019). PubMed: 31267313