Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit (ab213800)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit
See all Junctional Adhesion Molecule 1/JAM-A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 81pg/ml 5.83 7.2% 2 16 325pg/ml 16.9 5.2% 3 16 1280pg/ml 67.84 5.3% Inter-assay Sample n Mean SD CV% 1 24 73pg/ml 5.32 7.3% 2 24 310pg/ml 16.43 5.3% 3 24 1209pg/ml 93.09 7.7% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Junctional Adhesion Molecule 1/JAM-A Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213800) is designed for the quantitative detection of Human JAM-A in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for JAM-A has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: S28-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human JAM-A amount of sample captured in plate.
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Notes
Junctional adhesion molecule A/JAM-A is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that co-localizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration and a platelet receptor. JAM-A has a non-redundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human JAM-A coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human JAM-A antibody 1 x 100µl Lyophilized recombinant Human JAM-A standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Alternative names
- CD 321
- CD321
- CD321 antigen
see all -
Database links
- Entrez Gene: 50848 Human
- Omim: 605721 Human
- SwissProt: Q9Y624 Human
- Unigene: 517293 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213800 has been referenced in 1 publication.
- Lu J et al. MMP-9 as a Biomarker for Predicting Hemorrhagic Strokes in Moyamoya Disease. Front Neurol 12:721118 (2021). PubMed: 34531816